食品科学 ›› 2009, Vol. 30 ›› Issue (6): 188-190.doi: 10.7506/spkx1002-6630-200906042

• 分析检测 • 上一篇    下一篇

高效液相色谱法测定发酵液中表面活性素的含量

艾 嘉,陆兆新* ,别小妹,吕凤霞,袁勇军,黄现青   

  1. 南京农业大学食品科技学院
  • 收稿日期:2008-05-05 修回日期:2008-07-04 出版日期:2009-03-15 发布日期:2010-12-29
  • 通讯作者: 陆兆新 E-mail:fmb@njau.edu.cn
  • 基金资助:

    国家“863”计划项目(2006AA10Z346);国家自然科学基金项目(30671460;30871753)

High Performance Liquid Chromatography Analysis of Surfactin Content in Fermentation Broth of acillus amyloliquefaciens ES-2

AI Jia,LU Zhao-xin*,BIE Xiao-mei,LU Feng-xia,YUAN Yong-jun,HUANG Xian-qing   

  1. (College of Food Science, Nanjing Agricultural University, Nanjing 210095, China)
  • Received:2008-05-05 Revised:2008-07-04 Online:2009-03-15 Published:2010-12-29
  • Contact: LU Zhao-xin*, E-mail:fmb@njau.edu.cn

摘要:

建立一种以高效液相色谱法测定发酵液中表面活性素(surfactin)含量的新方法。采用COSMOSIL 5C18-AR-Ⅱ(4.6 × 250mm)色谱柱,以乙腈(含0.1%三氟乙酸)和水(含0.1%三氟乙酸)为流动相,流速0.84ml/min,梯度洗脱,紫外检测波长210nm,在20min 内检测到表面活性素。结果表明,运用本方法在1~100mg/L 范围内表面活性素浓度与积分面积之间具有良好的相关性(r=0.9998),精密度为1.5%,检出限为1mg/L,回收率达到91.36%。

关键词: 高效液相色谱, 发酵液, 表面活性素

Abstract:

A new analysis method of surfactin content in fermentation broth of Bacillus amyloliquefaciens ES-2 by high erformance liquid chromatography (HPLC) was established. The detection of surfactin was finished in 20 minutes at 210 nm n COSMOSIL 5C18-AR-Ⅱcolumn (4.6×250 mm) using acetonitrile (containing 0.1% triflouroacetic acid)-water (containing .1% triflouroacetic acid) (60:40, V/V) as mobile phase at the flow rate of 0.84 ml/min by gradient elution. The correlation oefficient between integral peak area and concentration is 0.9998 in the range of 1 to 100 mg/L. The precision, detection limit nd spike recovery of this method is 1.5%, 1 mg/L and 91.36%, respectively.

Key words: HPLC, ermentation broth, surfactin

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