食品科学 ›› 2009, Vol. 30 ›› Issue (5): 46-49.doi: 10.7506/spkx1002-6630-200905009

• 基础研究 • 上一篇    下一篇

非生物性因素对Nisin抗菌活性的影响

刘丽莉1,2,马美湖1,*,杨协力2   

  1. 1.华中农业大学食品科学技术学院 2.河南科技大学食品与生物工程学院
  • 收稿日期:2008-04-14 修回日期:2008-08-30 出版日期:2009-03-01 发布日期:2010-12-29
  • 通讯作者: 马美湖1,*, E-mail:mameihuhn@yahoo.com.cn
  • 基金资助:

    河南科技大学资助项目;“十一五”国家科技支撑计划项目(2006BAD05A16)

Effects of Abiotic Factors on Antibacterial Activities of Nisin against Staphylococcus aureus

LIU Li-li1,2,MA Mei-hu1,*, YANG Xie-li2   

  1. (1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;
    2. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003, China)
  • Received:2008-04-14 Revised:2008-08-30 Online:2009-03-01 Published:2010-12-29
  • Contact: MA Mei-hu1,*, E-mail:mameihuhn@yahoo.com.cn

摘要:

为了研究乳酸链球菌素(nisin)的抗菌活性,采用金黄色葡萄球菌为实验培养菌种,分别研究了不同的酸溶液(乙酸、硝酸、盐酸、柠檬酸)、不同的金属离子(Zn2+、K+、Fe3+、Cu2+)、不同的离子强度( 以Na + 的离子强度:0 、0.2、0.4、0.6、0.8mol/L)和不同的pH 值(4.0、4.5、5.0、5.5、6.0)等非生物性因素对Nisin 抗菌活性的影响。实验结果表明,乙酸对Nisin 的抗菌活性的提高效果最佳;加入金属离子会提高Nisin 的抗菌活性,其中K+ 的促进作用最强;离子强度的增加对Nisin 的抗菌活性有很大的提高;Nisin 在pH 4.0 的酸性环境中抗菌活性最好。

关键词: Nisin, 非生物性因素, 抗菌活性, 金黄色葡萄球菌

Abstract:

In this study, effects of various abiotic factors such as acid solutions (acetic acid, nitric acid, hydrochloric acid and citric acid), metal ions (Zn2+, K+, Fe3+ and Cu2+), ionic strength ( with Na+ ion intensity as the reference: 0, 0.2, 0.4, 0.6 and 0.8 mol/L) and pH value (4, 4.5, 5, 5.5 and 6) on the antibacterial activity of nisin against Staphylococcus aureus, a representative pathogeic bacterium in foods, were investigated. The results indicated that the effects of different acid solutions on the antibacterial activity of nisin are different, among which acetic acid shows the best improvement effects on the antibacterial activity of nisin; The addition of above metal ions can increase the antibacterial activity of nisin, among which K+ presents the strongest effect; The increase of ionic strength also can improve the antibacterial activity of nisin; Moreover, in the environment of pH 4.0 nisin plays the strongest antibacterial activity.

Key words: nisin, abiotic factor, antibacterial activity, Staphylococcus aureus

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