食品科学 ›› 2009, Vol. 30 ›› Issue (5): 72-75.doi: 10.7506/spkx1002-6630-200905015

• 基础研究 • 上一篇    下一篇

蜂王浆水溶性蛋白及其酶解产物的抗氧化活性

许建香1,张智武1,刘永东2,苏志国2,彭文君1   

  1. 1. 中国农科院蜜蜂研究所
    2.中国科学院过程工程研究所
  • 收稿日期:2008-05-13 修回日期:2008-08-30 出版日期:2009-03-01 发布日期:2010-12-29
  • 通讯作者: 张智武 E-mail:zhangzhiwu@yahoo.com
  • 基金资助:

    “十一五” 国家科技支撑计划项目(2006BAD06B04);中国农业科学院基本科研业务专项项目(JBKY0032007005CAAS)

Antioxidant Activities of Water Soluble Proteins in Royal Jelly and Their Hydrolysates

XU Jian-xiang1,ZHANG Zhi-wu1,*,LIU Yong-dong2,SU Zhi-guo2,PENG Wen-jun   

  1. (1.Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China;
    2.Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China)
  • Received:2008-05-13 Revised:2008-08-30 Online:2009-03-01 Published:2010-12-29
  • Contact: ZHANG Zhi-wu1,*, E-mail:zhangzhiwu@yahoo.com

摘要:

本研究分别采用清除二苯基-2- 苦肼自由基(DPPH·),抑制亚油酸氧化和总抗氧化能力测定等方法,探讨蜂王浆水溶性蛋白及其酶解产物的抗氧化特性。结果表明,蜂王浆水溶性蛋白(WSPs)本身的抗氧化活性很弱,但酶解后抗氧化活性增强,其中胃蛋白酶和胰蛋白酶共同酶解产物(PT-WSPs)的抗氧化活性最强。对WSPs 及其酶解产物进行膜分离后发现,分子量小于3kD,尤其是小于1kD 的肽类物质其清除DPPH·、抑制亚油酸氧化及总抗氧化能力最强。

关键词: 蜂王浆, 蛋白质, 酶解产物, 肽, 抗氧化活性

Abstract:

Through the measurement of total antioxidant capability (T-AOC), 1,1-diphenyl-2-picrylhydrazyl (DPPH·) radical scavening ability and inhibitory ability against oxidation of linoleic acid, the antioxidant activities of water soluble proteins (WSPs)in royal jelly and their hydrolysates were investigated. The results indicated that although WSPs have no obvious antioxidant activity, their activity significant increases after hydrolyzed, especially the product PT-WSPs from WSPs hydrolyzed by both pepsin and trypsin shows the highest antioxidant activity. Furthermore, the membrane separation of WSPs and their hydrolysates were conducted. It was discovered that small peptides with molecular weight of lower than 3 kD, especially lower than 1 kD, have powerful T-AOC, DPPH radical scavenging ability and inhibitory ability against oxidation of linoleic acid.

Key words: royal jelly, protein, hydrolysate, peptide, antioxidant activity

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