食品科学 ›› 2009, Vol. 30 ›› Issue (5): 175-178.doi: 10.7506/spkx1002-6630-200905040

• 基础研究 • 上一篇    下一篇

中国对虾过敏原基因特征的生物信息学分析

李振兴1,林 洪1 ,* ,王晓斐1,郭永超1,张轶群1,李小燕1,吕 朋2   

  1. 1.中国海洋大学水产品安全性实验室 2.山东省出入境检验检疫局
  • 收稿日期:2008-03-18 修回日期:2008-08-07 出版日期:2009-03-01 发布日期:2010-12-29
  • 通讯作者: 林洪 E-mail:linhong@ouc.edu.cn
  • 基金资助:

    国家“863”计划项目 (2006AA09Z427)

Bioinformatics Analysis of Allergen Gene Characteristics of Penneropenaeus chinensis

LI Zheng-xing1,LIN Hong1,*,WANG Xiao-fei1,GUO Yong-chao1,ZHANG Yi-qun1,LI Xiao-yan1,LU Peng2   

  1. (1.Seafood Safety Laboratory, Ocean University of China, Qingdao 266003, China;
    2.Entry-Exit Inspection and Quarantine Bureau of Shandong Province, Qingdao 266001, China)
  • Received:2008-03-18 Revised:2008-08-07 Online:2009-03-01 Published:2010-12-29
  • Contact: LIN Hong1,*, E-mail:linhong@ouc.edu.cn

摘要:

为了研究过敏原基因的基本性质、探讨过敏的机理,对中国对虾过敏原基因的部分序列进行克隆、测序,并分析该基因的有效密码子,碱基组成、密码子的偏好性,以及过敏原蛋白的氨基酸组成等性质。结果表明:中国对虾过敏原的部分基因序列长为1034,共编码264 个氨基酸,密码子的偏好性强,不同海产动物过敏原基因的有效密码子及碱基含量有很大的相似性。从基因序列和氨基酸序列的角度看,中国对虾的过敏原蛋白与其他种类海产甲壳动物的过敏原蛋白具有很强的相似性,这可能是导致海产品过敏原蛋白活性交叉反应的重要原因之一。

关键词: 虾, 过敏原基因, 过敏原蛋白, 生物信息学

Abstract:

To study gene characteristics of shrimp allergen and discuss the mechanism of food allergy, part of Chinese shrimp (Penneropenaeus chinensis) allergen gene was cloned, and its sequence, effective codes, base composition, codon preference as well as amino acid composition of allergen protein were determined. The results showed that the length of shrimp allergen gene is 1034 bp, the allergen protein is composed by 641 amino acids and the codon preference is high. According to the homology comparison between gene and amino acid sequences, the allergen protein of Chinese shrimp is highly similar to that of other sea crustaceans. This might be one of the important reasons to lead to the occur of activity cross reaction among allergen proteins in various seafoods.

Key words: shrimp, allergen gene, allergen protein, bioinformatics

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