食品科学 ›› 2009, Vol. 30 ›› Issue (5): 187-189.doi: 10.7506/spkx1002-6630-200905043

• 生物工程 • 上一篇    下一篇

地瓜生粉培养高产酵母菌诱变条件的实验研究

徐晓雪1,李润国2 ,* ,董国斌2,单红飞2   

  1. 1.中国医科大学生物芯片中心 2.沈阳师范大学化学与生命科学学院
  • 收稿日期:2008-04-11 修回日期:2008-09-01 出版日期:2009-03-01 发布日期:2010-12-29
  • 通讯作者: 李润国 E-mail:lirunguo@163.com
  • 基金资助:

    国家自然科学基金项目(30570445);辽宁省教育厅A 类攻关项目(2004D238)

Study on Mutation Conditions of High Alcohol-producing Yeast Fermenting Sweet Potato Meal Medium

XU Xiao-xue1,LI Run-guo2,*,DONG Guo-bin2,SHAN Hong-fei2   

  1. (1. Biochip Center of China Medical University, Shenyang 110001, China;
    2. College of Chemistry and Life Science, Shenyang Normal University, Shenyang 110034, China)
  • Received:2008-04-11 Revised:2008-09-01 Online:2009-03-01 Published:2010-12-29
  • Contact: LI Run-guo2,*, E-mail:lirunguo@163.com

摘要:

以地瓜生粉作为培养基的主要成分,为酵母菌的生长提供碳源,运用紫外线照射培养出的酵母菌,使其发生突变,经过初筛、复筛、驯化,培养出一株高产酒精的突变酵母菌S604(紫外线照射时间为60s,筛选4轮),并通过正交试验筛选出最优化培养基,在最优化培养基上培养突变菌株,测得其产酒精量比FCC2129 高。

关键词: 酵母菌, 诱变, 酒精, 正交实验

Abstract:

In this study, sweet potato meal was chosen as the principal constitute of culture medium to provide carbon source for the growth of yeast (Saccharomyces cerevisiaes). The mutation of original yeast was induced by UV radiation. After primary screening, secondary screening and domestication, a mutant of high alcohol-producing yeast S604 was obtained via 60-s UV radiation and 4-round screening. Then the culture medium composition of the mutant strain was optimized by orthogonal test. The fermntation experiment results showed that the alcohol yield by the mutant cultured in the optimized medium is higher than that by the original strain FCC2129.

Key words: yeast, sweet potato meal, mutation, alcohol, orthogonal test

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