食品科学 ›› 2009, Vol. 30 ›› Issue (5): 194-196.doi: 10.7506/spkx1002-6630-200905045

• 生物工程 • 上一篇    下一篇

Alcalase蛋白酶水解法制备山核桃肽的研究

李 勇,陈 光*   

  1. 吉林农业大学生命科学学院
  • 收稿日期:2008-04-27 修回日期:2008-06-10 出版日期:2009-03-01 发布日期:2010-12-29
  • 通讯作者: 陈光 E-mail:chg61@163.com

Study on Preparation of Walnut Peptide by Alcalase Hydrolysis Method

LI Yong,CHEN Guang*   

  1. (College of Life Science, Jilin Agricultural University, Changchun 130118, China)
  • Received:2008-04-27 Revised:2008-06-10 Online:2009-03-01 Published:2010-12-29
  • Contact: CHEN Guang* E-mail:chg61@163.com

摘要:

以脱脂山核桃渣为原料,利用Alcalase 蛋白酶对山核桃蛋白进行水解制备山核桃活性肽,以水解度(DH)表征其反应程度,确定其最佳水解条件为:温度55℃,pH9.0,底物浓度8%,酶质量分数6%,水解时间2.0h。在此条件下水解度(DH)为76.16%。

关键词: Alcalase蛋白酶, 山核桃, 活性肽

Abstract:

Walnut protein was hydrolyzed with alcalase to produce walnut peptide taking degree of hydrolysis (DH) to indicate reaction degree. The results showed that the optimum hydrolysis temperature, pH, substrate concentration, enzyme concentration and time are 55 ℃, 9.0, 8.0%, 6% and 2 h, respectively. Under the above conditions, the DH is up to 76.16%.

Key words: alcalase, walnut, active peptide

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