食品科学 ›› 2009, Vol. 30 ›› Issue (5): 197-200.doi: 10.7506/spkx1002-6630-200905046

• 生物工程 • 上一篇    下一篇

噬菌体对荧光假单胞菌A46的感染及其防治的初步研究

张志平1,李晓明1,孙文敬1 ,2,王 多1,刘敬泽1 ,*   

  1. 1.河北师范大学生命科学学院 2. 江苏大学食品与生物工程学院
  • 收稿日期:2008-04-28 修回日期:2008-09-02 出版日期:2009-03-01 发布日期:2010-12-29
  • 通讯作者: 刘敬泽 E-mail:liujingze@mail.hebtu.edu.cn
  • 基金资助:

    2007 年商务部共性技术研发项目(商产函[2007]80 号)

Preliminary Study on Bacteriophage Infection of Pseudomonas fluorescens A46 and Corresponding Control

ZHANG Zhi-ping1,LI Xiao-ming1,SUN Wen-jing1,2,WANG Duo1,LIU Jing-ze1,*   

  1. (1. College of Life Sciences, Hebei Normal University, Shijiazhuang 050016, China;
    2. College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China)
  • Received:2008-04-28 Revised:2008-09-02 Online:2009-03-01 Published:2010-12-29
  • Contact: LIU Jing-ze1,* E-mail:liujingze@mail.hebtu.edu.cn

摘要:

利用透射电镜观察2- 酮基-D- 葡萄糖酸产生菌荧光假单胞菌A46 感染噬菌体KS461-2 后的菌体形态变化,测定该菌株耐噬菌体突变频率,考察不同pH 值对噬菌体增殖的影响,检测4 种常用药物对噬菌体的防治效果。结果表明,随着噬菌体感染时间的延长,菌体形态变化差异显著;该菌株耐噬菌体KS461-2 的突变频率在2.5 × 10-6左右;噬菌体在pH7.5~8.0 的条件下容易吸附于宿主而增殖;草酸铵能有效抑制该噬菌体的增殖,而对宿主菌的生长无明显影响,最佳添加浓度为0.7%。

关键词: 荧光假单胞菌, 2-酮基-D-葡萄糖酸, 噬菌体, 感染, 菌体形态

Abstract:

The changes of cell morphology of Pseudomonas fluorescens A46, a strain producing 2-keto-D-gluconic acid, were observed by transmission electron microscope after infected by bacteriophage KS461-2. The mutation frequency of P. fluorescens A46 resisting the bacteriophage was determined, the multiplication of the bacteriophage was studied at different pH values, and the control effects of four medicaments on the bacteriophage infection were investigated. The current results showed that the morphology significantly changes following with infection time extending, the mutation frequency of P. fluorescens is about 2.5×10-6, the bacteriophage easily adheres to the host bacteria and proliferates in the pH range of 7.5 to 8.0, and ammonium oxalate can remarkably restrain the multiplication of the bacteriophage and does not affect the growth of P. fluorescens A46, especially the addition of 0.7% is the best.

Key words: Pseudomonas fluorescens, 2-keto-D-gluconic acid, bacteriophage, infection, cell morphology

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