食品科学 ›› 2009, Vol. 30 ›› Issue (5): 233-238.doi: 10.7506/spkx1002-6630-200905054

• 营养卫生 • 上一篇    下一篇

大球盖菇多糖清除自由基活性和对D-半乳糖氧化损伤小鼠的抗氧化作用

王峰1,王晓炜1,陶明煊1,程光宇2.3,杨启银2,曩潇潇2   

  1. 1. 南京师范大学金陵女子学院
    2. 南京师范大学生命科学学院 3.江苏吴中大自然生物工程有限责任公司
  • 收稿日期:2008-03-14 修回日期:2008-05-23 出版日期:2009-03-01 发布日期:2010-12-29
  • 通讯作者: 程光宇 E-mail:mxwcgy@yahoo.com.cn
  • 基金资助:

    江苏省高校自然科学基础研究项目(07KJD550116)

Free Radicals Scavenging Activity of Stropharia rugoso-annulata Polysaccharides and Their Protective Effects on D-galactose-induced Oxidative Damage of Mice

WANG Feng1,WANG Xiao-wei1,TAO Ming-xuan1,CHENG Guang-yu2,3,*,YANG Qi-yin2,NANG Xiao-xiao2   

  1. (1.Ginling College, Nanjing Normal University, Nanjing 210097, China;2.School of Life Science, Nanjing Normal University,
    Nanjing 210097, China;3. Jiangsu Wuzhong Nature Biotech Co. Ltd., Nanjing 210097, China)
  • Received:2008-03-14 Revised:2008-05-23 Online:2009-03-01 Published:2010-12-29
  • Contact: CHENG Guang-yu2,3,*, E-mail:mxwcgy@yahoo.com.cn

摘要:

目的:探讨大球盖菇多糖(SRP)体外自由基清除活性和对D- 半乳糖所致氧化损伤小鼠的抗氧化作用。方法:SRP 体外抗氧化活性用自由基清除能力、红细胞溶血作用、肝匀浆MDA 生成作用和抑制线粒体肿胀的方法来测定;在动物实验中,通过连续60d 颈背部皮下注射D- 半乳糖建立氧化损伤动物模型,在造模的同时给予低、中、高剂量(100、200、400mg/kg·d) SRP,并定期测定血液SOD 活性和MDA 含量,动物被处死后测定血液和肝脏的抗氧化指标。结果:SRP 在体外清除超氧自由基IC50 为132.5μg/ml,在583.1μg/ml 浓度时对羟自由基清除率为47.44%,在832μg/ml 时对红细胞氧化溶血抑制率为23.8%,在500μg/ml 时抑制线粒体肿胀程度与对照组相同,在537μg/ml 时在自氧化和诱导氧化条件下,抑制肝匀浆MDA 的生成分别为38.0% 和40.9%;在动物实验中,SRP 对小鼠体重变化无显著影响,能使降低的血红蛋白(Hb)含量和肝指数恢复到正常水平给予SRP(200mg/kg)在20、45、60d 时能提高血液SOD 活性,在45、60d 时能降低血液MDA 含量,与模型组比差异有显著性;在小鼠血清抗氧化指标中,模型组MDA 含量升高,谷胱甘肽过氧化物酶(GPx)和总抗氧化能力(TAOC)水平降低,与对照组比差异有显著性,给予SRP 后MDA 含量显著降低,GPx 活性和TAOC 显著提高。与模型组比,SRP 组的肝抗氧化酶(总SOD、MnSOD、CuZnSOD 及GPx)活性及TAOC 显著提高,MDA 含量显著下降,差异有显著性。结论:SRP 能有效地清除自由基,对D- 半乳糖所致氧化损伤小鼠血液和肝脏有显著的改善作用,说明SRP 体内和体外具有很好的抗氧化作用。

关键词: 大球盖菇, 多糖, 自由基, 氧化损伤模型, 抗氧化作用

Abstract:

Objective: To investigated antioxidant activities of Stropharia rugoso-annulata polysaccharides (SRP) in vivo and in vitro, respectively. Methods: The antioxidant activities of SRP in vitro were evaluated by determining its ability to scavenge free radicals and inhibit erythrocyte hemolysis, MDA generation and mitochondria swelling. In animal test, the oxidant damage model was produced by consecutively injecting D-galactose into the hypoderm of mice for 60 days. At the same time the mice were administered orally with three dosages of SRP (100, 200 and 400 mg/kg·b, respectively), and the levels of SOD and MDA in blood were regularly measured. After mice were sacrificed, their blood and liver samples were collected for assaying the antioxidant indexes. Results: in vitro SRP shows excellent antioxidant activities. The IC50 values of SRP to scavenge superoxide radicals is 132.5 μg/ml, the scavenging rate of 583.1 μg/ml SRP to hydroxyl radicals is 47.44%, the inhibitory rate of 832 μg/ml SRP to erythrocyte homolysis induced by H2O2 is 23.8%, the inhibitory rate of 500 μg/ml SRP to mitochondria swelling induced by Fe2++VC is as good as the control group, and the inhibitory rates of 537 μg/ml SRP to liver MDA generation under autoxidation and induced oxidation conditions are 38% and 40.8% respectively. In the animal test, SRP treatment has no effect on the body weight of model mice, but makes the decreased hemoglobin (Hb) content and liver index in model mice recover to the normal range. Compare with the model group, SRP (200 mg/kg) treatment significantly promotes blood SOD activity on the 20th, 40th, 60th day and reduces blood MDA content on the 40th and 60th day after administering. Moreover, SRP treatment significantly reduces serum and liver MDA contents and promotes activities of serum GPx and TAOC and liver endogenous antioxidant enzymes (total SOD, Cu,Zn-SOD, Mn-SOD, GPx and TAOC) of model mice. Conclusion: SRP possesses excellent antioxidant activities of scavenging free radicals in vitro and D-galactose induced oxidative damage in vivo.

Key words: Stropharia rugoso-annulata polysaccharides (SRP), free radical, oxidative damage model, antioxidation

中图分类号: