食品科学 ›› 2009, Vol. 30 ›› Issue (4): 204-206.doi: 10.7506/spkx1002-6630-200904043

• 分析检测 • 上一篇    下一篇

高效液相色谱柱前衍生法测定蜜饯中的甜蜜素

何良兴1,林赛君2,柯春晖1,郭柏坤1   

  1. 1.杭州市余杭区质量计量监测中心 2.杭州市质量技术监督检测院
  • 收稿日期:2008-02-27 修回日期:2008-05-20 出版日期:2009-02-15 发布日期:2010-12-29
  • 通讯作者: 郭柏坤 E-mail:gbkstarfish@yahoo.com.cn

Determination of Cyclamate in Preserved Fruits by High-performance Liquid Chromatography Coupled with Pre-column Derivatization

HE Liang-xing1,LIN Sai-jun2,KE Chun-hui1,GUO Bai-kun1   

  1. 1. Supervising and Testing Center for Quality and Measurement of Yuhang District of Hangzhou, Hangzhou 311199, China ;
    2. Hangzhou Institute of Test and Calibration for Quality and Technology Supervision, Hangzhou 301110, China
  • Received:2008-02-27 Revised:2008-05-20 Online:2009-02-15 Published:2010-12-29
  • Contact: GUO Bai-kun E-mail:gbkstarfish@yahoo.com.cn

摘要:

目的:建立简便、准确、灵敏的测定蜜饯中甜蜜素含量方法。方法:在酸性介质条件下,溶液中的甜蜜素同次氯酸钠溶液发生反应,其衍生产物在紫外-HPLC 法下测定。结果:方法最低检出限为0.05g/kg,其在0.02~1.00mg/ml 范围内呈线性关系,相关系数为0.9995,方法的相对标准偏差为1.56%~4.49%,回收率为91.5%~98.5%,检测结果与GB/T5009.97 — 2003 气相色谱法无显著差异。结论:本法灵敏度高,操作简单,稳定性和重复性好,适合于蜜饯中甜蜜素含量的测定。

关键词: 甜蜜素, 高效液相色谱, 柱前衍生

Abstract:

Objective: to establish a simple, accurate and sensitive method for determination of cyclamate in preserved fruits by high-performance liquid chromatography with pre-column derivatization. Method: Through the reaction between cyclamate and sodium hypochlorite under acid medium, N,N-dichloro- cyclohexylamine was determined by UV-HPLC. Results: The detection limit is 0.05 g/kg. The calibration curve is linear in the range of 0.02 to 1.00 mg/ml with a correlation coefficient of 0.9995. The relative standard deviation is 1.56% to 4.49% and the recovery rate is in the range of 91.5% to 98.5%. The determination result has no significant deference from that by the method of GB5009.97 — 2003. Conclusion: The method is sensitivity, simple and stable and has good repetition. It can be used for the determination of cyclamate in preserved fruit.

Key words: cyclamate, high-performance liquid chromatography, pre-column derivatization

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