食品科学 ›› 2009, Vol. 30 ›› Issue (3): 36-38.doi: 10.7506/spkx1002-6630-200903006

• 基础研究 • 上一篇    下一篇

中草药提取物对链格孢菌抑制作用增效组合的研究

吴振宇1,艾启俊2 ,*,王 燕1,魏月琴1,哈斯格根3,张 伟1   

  1. 1.北京农学院植物科学技术系 2.北京农学院食品科学系 3.内蒙古农业大学食品科学与工程学院
  • 收稿日期:2008-01-09 修回日期:2008-02-24 出版日期:2009-02-01 发布日期:2010-12-29
  • 通讯作者: 艾启俊 E-mail:aqjbnf@sohu.com
  • 基金资助:

    “十一五”国家科技支撑计划项目(2006BAD22B0114); 北京市科委项目(D0706003040291)

Study on Combined Inhibitory Effects of Chinese Herb Extracts against Alternaria alternate

WU Zhen-yu1,AI Qi-jun2,*,WANG Yan1,WEI Yue-qin1,Hasigegen3,ZHANG Wei1   

  1. (1.Department of Plant Science and Technology, Beijing University of Agriculture , Beijing 102206, China;
    2.Department of Food Science, Beijing University of Agriculture, Beijing 102206, China;3.College of Food Science
    and Engineering, Inner Mongolia Agricultural University, Huhhot 010018, China)
  • Received:2008-01-09 Revised:2008-02-24 Online:2009-02-01 Published:2010-12-29
  • Contact: AI Qi-jun2,* E-mail:aqjbnf@sohu.com

摘要:

为寻找天然果蔬防腐剂,本实验在离体条件下,采用生长速率法测定了鹿蹄草素、黄连、黄柏、厚朴4 种中草药提取物对梨链格孢菌的抑制作用,并经线性回归计算50% 抑制时的有效浓度(EC50)分别为0.22、10.19、10.35、0.34mg/ml。根据EC50,用等效线法相加作用线的6 等分点设置配比,共毒因子法判断增效标准。结果表明,各种配比的混配剂中黄连+厚朴为最佳增效作用组合,并以25:2 增效作用最显著。

关键词: 中草药提取物, 链格孢菌, 抑制作用

Abstract:

Agar plate tests were conducted to test the inhibitory effects in-vitro of Chinese herb extracts such as pyrolin, Coptis chinensis Franch extract, Phellodendron chinense Schneid extract and Magnolia officinalis Rehd extract against Alternaria alternate in order to find the new type natural antisepic agent. Based on linear regression, it was calculated that the 50% of effective concentration (EC50) of these extracts are 0.22, 10.19, 10.35, 0.34 mg/ml respectively. According to EC50, five ratios were determined by six equal-part points of additive action line, and synergism was identified by co-toxicity factor method. The result showed that the extracts of Coptis chinensis Franch and Magnolia officinalis Rehd with the ratio of 25:2 are the best mixture for controlling A. alternate.

Key words: Chinese herb extract, Alternaria alternate, inhibitory effect

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