食品科学 ›› 2009, Vol. 30 ›› Issue (3): 145-149.doi: 10.7506/spkx1002-6630-200903032

• 生物工程 • 上一篇    下一篇

模拟微重力效应反应器培养螺旋藻的研究

迟海洋1,2,张奕婷1,2,谯顺彬3,张义明2,3,*   

  1. 1.广东食品药品职业学院 2.贵州省发酵工程与生物制药重点实验室 3.贵州科技工程职业学院
  • 收稿日期:2008-01-17 修回日期:2008-05-15 出版日期:2009-02-01 发布日期:2010-12-29
  • 通讯作者: 张义明 E-mail:ymzhang5262@163.com
  • 基金资助:

    国家自然科学基金项目(30460039);教育部“春晖计划”项目;贵州省科技攻关项目(2004GGT022);
    贵州省优秀科技教育人才省长专项基金资助项目(黔科教办200304 号)

Study on Culture of Spirulina platensis with Simulated Microgravity Reactor

CHI Hai-yang1,2,ZHANG Yi-ting1,2,QIAO Shun-bin1,2,ZHANG Yi-ming2,3,*   

  1. (1.Guangdong Food and Drug Vocational College, Guangzhou 5105201, China;
    2. Guizhou Provincal Key Laboratory of Fermentation Engineering and Biopharmacy, Guiyang 550003, China;
    3. Guizhou Technological Engineering and Vocational College, Guiyang 550008, China)
  • Received:2008-01-17 Revised:2008-05-15 Online:2009-02-01 Published:2010-12-29
  • Contact: ZHANG Yi-ting1,2 E-mail:ymzhang5262@163.com

摘要:

本实验主要介绍了自制模拟微重力效应反应器(回转器)的工作原理、结构和特征,并用其进行螺旋藻光合自养分批培养,同时与摇瓶和发酵罐式光生物反应器对比。研究结果表明:在接种量为10%、光照强度为4000lx、温度为25℃的条件下培养9d,模拟微重力效应反应器效果最好,细胞干重为0.91g/L,是螺旋藻大规模培养的2.41 倍,并且螺旋藻具有较好的细胞形态。

关键词: 螺旋藻, 失重模拟, 光合自养, 分批培养, 回转器

Abstract:

In this study, the operation principle, structure and feature of simulated microgravity reactor were introduced mainly. Spirulinas platensis were cultured in phototrophic batches in three kinds of culture devices. The results showed that the simulated microgravity reactor (clinostat) for Spirulinas platensis culture is better than shake flask and fermenter light bioreactor, and its optimum conditions are as following: inoculation concentration 10%, illumination 4000 lx, 25 ℃ and culture time 9 days. By this way, the production of Spirulina platensis (dw) is 0.91 g/L, which is 2.41 times as much as that by large-scale culture and the Spirulina platensis trichomes cultured in the simulated microgravity reactor have good cell morphology.

Key words: Spirulina platensis, weightlessness simulation, phototrophic batch culture, clinostat

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