食品科学 ›› 2009, Vol. 30 ›› Issue (3): 159-162.doi: 10.7506/spkx1002-6630-200903036

• 生物工程 • 上一篇    下一篇

Candida shehatae 的纯化及其利用木糖和葡萄糖发酵的特性研究

汤 斌,周逢云,张庆庆,翟光雯,陈阿娜   

  1. 安徽工程科技学院生化工程学,微生物发酵安徽省工程技术研究中心
  • 收稿日期:2008-01-28 修回日期:2008-06-04 出版日期:2009-02-01 发布日期:2010-12-29
  • 通讯作者: 周逢云 E-mail:zfy1116@126.com
  • 基金资助:

    安徽省高校自然科学基金重点项目(KJ2007A018)

Screening of Candida shehatae TZ8-13 Converting Xylose and Glucose into Ethanol Efficiently and Its Fermentation Characteristics

TANG Bin,ZHOU Feng-yun,ZHANG Qing-qing,ZHAI Guang-wen,CHEN A-na   

  1. (Department of Biochemical Engineering, Anhui University of Technology and Science, Anhui Provincial Engineering Technology
    Research Center of Microbial Fermentation, Wuhu 241000, China)
  • Received:2008-01-28 Revised:2008-06-04 Online:2009-02-01 Published:2010-12-29
  • Contact: ZHOU Feng-yun E-mail:zfy1116@126.com

摘要:

通过对休哈塔假丝酵母TZ1 分离纯化以获取高产乙醇菌株,并对木糖、葡萄糖以及不同比例的混合糖发酵产乙醇进行实验研究。结果显示,筛选出的TZ8-13 对木糖和葡萄糖都有良好的发酵性能,糖浓度为60g/L 时的乙醇产量分别达到了21.6g/L 和24.2g/L,糖利用率分别为97.81%(72h)和99.13%(36h),较初始菌株TZ1 发酵木糖乙醇产量提高了55.38%、木糖利用率提高了19.54%;混合糖发酵存在糖的二次利用关系,TZ8-13 首先利用葡萄糖。木糖和葡萄糖比为1:1 时乙醇产量为22.8g/L。

关键词: 休哈塔假丝酵母TZ8-13, 木糖, 葡萄糖, 乙醇

Abstract:

An excellent strain, Candida shehatae TZ8-13, was attained by separating and purifying from initial Candida shehatae TZ1. Xylose, glucose and glucose-xylose mixtures ( at 60 g/L of total sugar) were fermented by the strain TZ13 for producing ethanol. The results indicated that xylose and glucose can be fermented efficiently by the strain TZ8-13. The ethanol yields are 21.6 g/L and 24.2 g/L respeetively when the concentrations of both xylose and glucose are 60 g/L, and the utilization rates of xylose and glucose are up to 97.81% (72 h) and 99.13% (36 h), respectively. The ethanol yield and utilization rates of xylose as sole carbon source of the screened strain TZ13 are increased by 55.38% and 19.54%, repectively, compared with the initial strain TZ1. Glucose as carbon source is prior to xylose. When the concentration ratio of glucose to xylose is 1, the ethanol yield is 22.8 g/L.

Key words: Candida shehatae TZ8-13, xylose, glucose, ethanol

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