食品科学 ›› 2009, Vol. 30 ›› Issue (2 ): 193-195.doi: 10.7506/spkx1002-6630-200902042

• 分析检测 • 上一篇    下一篇

HPLC法测定咸蛋、皮蛋黄中的四种苏丹红染料

王明月1,桂卫星2,袁宏球1   

  1. 1.中国热带农业科学院分析测试中心 2.南昌市蔬菜科学研究所
  • 收稿日期:2008-01-24 修回日期:2008-03-22 出版日期:2009-01-15 发布日期:2010-12-29
  • 通讯作者: 王明月 E-mail:guihongxing@263.net

High Performance Liquid Chromatography for Determination of Sudan Dyes in Salted and Preserved Eggs

WANG Ming-yue1,GUI Wei-xing2,YUAN Hong-qiu1   

  1. (1. Analysis and Testing Centre, Chinese Academy of Tropica Agricultural Sciences, Haikou 571101, China ;
    2. Nanchang Vegetable Science Research Institute, Nanchang 330001, China)
  • Received:2008-01-24 Revised:2008-03-22 Online:2009-01-15 Published:2010-12-29
  • Contact: WANG Ming-yue1 E-mail:guihongxing@263.net

摘要:

采用高效液相色谱(HPLC)法测定咸蛋、皮蛋中的四种苏丹红染料。测定结果表明,苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ在0~0.5mg/L 时其峰面积与进样质量浓度呈良好的线性关系,其线性相关系数为0.9999、0.9995、0.9999 和0.9999(n=6),加标样品测定的相对标准偏差(RSD)为1.1%、2.2%、1.9% 和2.4%(n=6)。苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ的最小检出限均为10μg/kg。苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ回收率均为90%~105%。结果显示:该方法快速、灵敏、可靠,具有简便、重现性好的特点。

关键词: 咸蛋, 皮蛋, 苏丹红Ⅰ, 苏丹红Ⅱ, 苏丹红Ⅲ, 苏丹红Ⅳ, 高效液相色谱法

Abstract:

The method for separation and determination of Sudan dyes in salted egg and preserved egg by reversed-phase high performance liquid chromatography was developed using chromatography conditions. The results showed that Sudan dyes have good linearity in the range from 0 to 0.5 mg/L, the correlation coefficients are 0.9999, 0.9995, 0.9999 and 0.9999 (n=6) respectively for four Sudan dyes. The relative standard deviations of spiked sample are 1.1%, 2.2%, 1.9% and 2.4% (n=6). The detection limits of all four Sudan dyes are 10μg/ kg. The average recovery of four Sudan dyes is 95% to 105%. In conclusion, this method is rapid, sensitive, simple, accurate and reproducible.

Key words: salted egg, preserved egg, Sudan dye Ⅰ, Sudan dye Ⅱ, Sudan dye Ⅲ, Sudan dye Ⅳ, high performance liquid chromatography (HPLC)

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