食品科学 ›› 2009, Vol. 30 ›› Issue (1): 177-180.doi: 10.7506/spkx1002-6630-200901042

• 生物工程 • 上一篇    下一篇

蛹虫草多糖对紫外线诱发蚕豆根尖细胞微核的影响

朱蕴兰1,邵 颖2,陈安徽2,陈宏伟2 ,*   

  1. 1.徐州工程学院环境工程学院 2.徐州工程学院食品工程学院
  • 收稿日期:2007-12-10 修回日期:2008-05-20 出版日期:2009-01-01 发布日期:2010-12-29
  • 通讯作者: 陈宏伟 E-mail:chenhw@xzit.edu.cn
  • 基金资助:

    江苏省“六大人才高峰”资助项目;“3 3 3 工程培养对象”资助项目;
    江苏省高校科研计划指导项目(05KJD180203)

Effects of Cordyceps militaris Polysaccharides on Ultraviolet-induced Micronucleus Rate of Vicia faba Root-tip Cells

ZHU Yun-lan1,SHAO Ying2,CHEN An-hui2,CHEN Hong-wei2,*   

  1. (1.College of Environmental Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China;
    2.College of Food Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China)
  • Received:2007-12-10 Revised:2008-05-20 Online:2009-01-01 Published:2010-12-29
  • Contact: CHEN Hong-wei2 E-mail:chenhw@xzit.edu.cn

摘要:

目的:研究蛹虫草多糖对紫外线诱发的蚕豆根尖细胞微核的影响。方法:采用蚕豆根尖细胞微核试验。结果:浓度为30、50、80、100μg/ml 的蛹虫草胞内、胞外多糖对蚕豆根尖微核率与阳性对照组相比均具有极显著差异(p < 0.01),浓度为100μg/ml 的蛹虫草胞内、胞外多糖对根尖微核的抑制率分别为47.68%、43.04%。蛹虫草胞内、胞外多糖每种样品的不同浓度之间对微核的影响存在极显著的差异(p < 0.01),且蚕豆微核率随多糖浓度的提高而降低。结论:蛹虫草胞内、胞外多糖均可有效抑制紫外线诱发的蚕豆根尖细胞微核的产生。

关键词: 蛹虫草, 多糖, 微核, 紫外线

Abstract:

Micronucleus test was designed to study effects of Cordyceps militaris polysaccharides on micronucleus rate of Vicia faba root-tip cells induced by ultraviolet (UV). The results showed that the inhibition rates of Cordyceps militaris endopolysaccharides and exopolysaccharides at the concentrations of 30 , 50 , 80, and 100 μg/ml respectively on micronucleus of Vicia faba root-tip cells induced by UV are lower obviously than the positive control group. When the concentration of both endopolysaccharides and exopolysaccharides is 100 μg/ml, their inhibition rates against micronucleus reach 47.68 % and 43.04 % respectively. There are significant differences among the effects of four concentrations of Cordyceps militaris polysaccharides on micronucleus (p < 0.01). In addition, with the increase of the concentration of polysaccharides, the micronucleus rate of Vicia faba root-tip cells decreases. In conclusion, endopolysaccharides and exopolysaccharides from Cordyceps militaris could inhibit the yield of micronucleus of Vicia faba root-tip cells induced by UV.

Key words: Cordyceps militaris, polysaccharides, micronucleus, ultraviolet (UV)

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