食品科学 ›› 2011, Vol. 32 ›› Issue (3 ): 40-45.doi: 10.7506/spkx1002-6630-201103011

• 基础研究 • 上一篇    下一篇

CMC与CMC-Na 改性鸡蛋清蛋白溶胶- 凝胶性质研究

徐保立,李斌 ,朱波,夏 莹,范劲松,郑晓东,蔡 杰,马美湖   

  1. 华中农业大学食品科学技术学院,湖北武汉 430070
  • 收稿日期:2010-05-24 修回日期:2011-01-07 出版日期:2011-02-15 发布日期:2011-01-13
  • 通讯作者: 李斌 E-mail:libinfood@yahoo.com.cn
  • 基金资助:

    国家“863”计划项目(2007AA10Z310;2007AA100408);教育部新世纪优秀人才项目(NECT-2007073);湖北省杰出青年人才基金项目(2007ABB016)

Properties Improvement of Egg Albumen by Conjugation with CMC or CMC-Na

XU Bao-li,LI Bin,ZHU Bo,XIA Ying,FAN Jin-song,ZHENG Xiao-dong,CAI Jie,MA Mei-hu   

  1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2010-05-24 Revised:2011-01-07 Online:2011-02-15 Published:2011-01-13
  • Contact: LI Bin E-mail:libinfood@yahoo.com.cn

摘要: 将一定质量分数的离子型多糖羧甲基纤维素(CMC)与羧甲基纤维素钠(CMC-Na)分别溶于蛋清中,冷冻干燥后,将得到的样品置于相对湿度为75% 的环境中干热反应一定时间后,通过质构分析、色度分析、SDS-PAGE、差示扫描量热分析、扫描电子显微镜研究鸡蛋清蛋白与CMC 及CMC-Na 交联改性后性质的变化。结果表明:反应温度60℃、反应时间2d 时,各样品的凝胶强度显著增大,凝胶的微观网络更为均匀致密,蛋清粉中的小分子质量蛋白质减少。证明适度的反应可增强蛋清粉的凝胶强度,但随时间延长,过度反应则会造成分子质量过大、溶解度下降,色泽显著加深。起泡性的分析表明:各反应2d 后样品的起泡性均明显增强,其中以60℃,添加0.25% 的CMC 和CMC-Na 的起泡力最强,分别增大了0.6 倍和0.5 倍,泡沫稳定性亦明显增强。证明在此反应条件下,可获得同时兼顾较高凝胶性和较高起泡性的新型蛋白粉。

关键词: 蛋清蛋白, 羧甲基纤维素, 交联反应, 凝胶强度

Abstract: Egg white was blended with sodium carboxymethyl cellulose (CMC-Na) or carboxymethyl cellulose (CMC) of certain concentration respectively, and the dry-heated products were obtained at relative humidity (75%) after freeze drying. The products were analyzed on gel strength, color scale, scanning electron microscope observation, SDS-PAGE, and Differential Scanning Calorimetric (DSC) at intervals. The results showed that a significant increase in the gel strength of egg white, more uniform and compact gel network structure and a significant reduction in the content of proteins with small molecular weight were found after 2 days of reaction at 60 ℃. The foaming capacity of egg albumen was found to be enhanced significantly by 2-day-long reaction, but the excessive cross-linking might lead to a large molecular weight, decrease the solubility of egg white powder and darken its color. Adding CMC or CMC-Na at a concentration of 0.25% resulted in the best foaming capacity at 60 ℃, with 0.6-fold and 0.5-fold increases in comparison with a blank sample, respectively and meanwhile, a significant improvement was observed for foam stability. It was proved that a novel egg white powder with both higher gel property and foaming capacity can be obtained under the reaction conditions.

Key words: egg albumen, carboxymethyl cellulose, crosslinking reaction, gel strength

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