食品科学 ›› 2011, Vol. 32 ›› Issue (3 ): 95-97.doi: 10.7506/spkx1002-6630-201103023

• 基础研究 • 上一篇    下一篇

唐古特白刺果实提取物抗氧化活性评价

任旭,陈贵林*   

  1. 内蒙古大学生命科学学院,内蒙古呼和浩特 010021
  • 收稿日期:2010-06-01 修回日期:2011-01-09 出版日期:2011-02-15 发布日期:2011-01-13
  • 通讯作者: 陈贵林 E-mail:guilinchen@yahoo.com.cn;chengl@life.imu.edu.cn
  • 基金资助:

    国家自然科学基金项目(30660015);高等学校博士学科点专项科研基金项目(20091501110002);内蒙古自治区科技创新引导奖励资金(2010年度)资助项目

Antioxidant Activity of Extracts from Nitraria tangutorum Bohr. Fruits

REN Xu,CHEN Gui-lin   

  1. College of Life Science, Inner Mongolia University, Hohhot 010021, China
  • Received:2010-06-01 Revised:2011-01-09 Online:2011-02-15 Published:2011-01-13

摘要: 采用95% 乙醇加热回流提取唐古特白刺(Nitraria tangutorum Bohr.)果实成分,依次用石油醚、氯仿、乙酸乙酯和正丁醇进行萃取。用DPPH 自由基法和ABTS+·法进行抗氧化活性实验,并选取抗坏血酸(VC)和生育酚(VE)作为对照。结果显示乙酸乙酯提取物(EAF)具有较高的抗氧化性,进一步对其进行硅胶柱层析分离,得到6 个组分(EAF-Ⅰ~EAF- Ⅵ),其中EAF-Ⅰ和EAF- Ⅳ的抗氧化性均高于VC 和VE。白刺果实提取物具有良好的抗氧化性,有进一步开发为天然抗氧化剂的应用前景。

关键词: 唐古特白刺果实, DPPH 自由基, ABTS+·, 抗氧化性, 分离提取

Abstract: Nitraria tangutorum Bohr. fruits were extracted using 95% ethanol, and then the extract was partitioned with petroleum ether, chloroform, ethyl acetate and n-butanol successively. The antioxidant activities of different fractions were evaluated by scavenging capability of ABTS+ and DPPH free radicals, and compared with the antioxidant activities of ascorbic acid (vitamin C) and tocopherol (vitamin E). Results indicated that ethyl acetate fraction (EAF) exhibited the highest antioxidant activity. EAF was further fractionated into 6 fractions by silica gel column chromatography. The antioxidant activities of EAFI and EAF-IV were higher than those of vitamin C and vitamin E. Therefore, N. tangutorum fruit extracts have excellent antioxidant activities, and have promising application prospects as a natural antioxidant.

Key words: Nitraria tangutorum Bohr. fruit, DPPH free radical, ABTS+ free radical, antioxidant activity, separation and purification

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