食品科学 ›› 2011, Vol. 32 ›› Issue (3 ): 126-129.doi: 10.7506/spkx1002-6630-201103030

• 生物工程 • 上一篇    下一篇

有机和常规红提葡萄附生菌的比较分析

刘万振,武婷婷,生吉萍,申琳   

  1. 中国农业大学食品科学与营养工程学院
  • 收稿日期:2010-08-10 修回日期:2010-12-30 出版日期:2011-02-15 发布日期:2011-01-13
  • 基金资助:

    国家自然科学基金项目(30972065;30671471);国家公益性行业(农业)科技项目(200803033);“十一五”国家科技支撑计划项目(2006BAD22B07-01;2007BAC15B05-04;2008BADAIB07)

Comparative Analysis of Epiphyte between Organic and Common Red Globe Grapes

LIU Wan-zhen,WU Ting-ting,SHENG Ji-ping,SHEN Lin*   

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Received:2010-08-10 Revised:2010-12-30 Online:2011-02-15 Published:2011-01-13

摘要: 以伊犁金山葡萄庄园的红提葡萄为原料,通过比较常规葡萄和有机葡萄不同器官表面附生菌群的差异,旨在揭示常规和有机两种不同栽培模式对葡萄附生菌群的影响。葡萄附生菌的分离、筛选方法为平板培养法,微生物的计数方法为最大或然数法。结果显示:葡萄根部附生菌的群落总数最大(105CFU/mm2),茎次之,果实最小(约102CFU/mm2)。在果实上,有机葡萄附生真菌种类多于常规葡萄,而附生细菌种类与之相反。除葡萄叶没有显著差异外,有机葡萄各器官(根、茎、果实)附生菌菌落总数均大于常规葡萄。初步表明,有机栽培可以更好地保持生态环境中微生物群落的生物多样性。

关键词: 红提葡萄, 有机食品, 附生菌, 食品安全

Abstract: Both organic and conventional red globe grape were sampled from Jinshan vineyard in Yili. The effect of different farming models of conventional and organic farming models on the epiphyte distribution was studied preliminarily in current paper by comparing the epiphytic biodiversity in grape organs. The agar-plate culture method was used for the microbial isolation, and the most-probable-number (MPN) was used for the enumeration of epiphyte population. The result was as follows: the largest number of epiphytes located on the root (up to 105 CFU/mm2), and the stem took second place, and the smallest on fruit (about 102 CFU/mm2). Besides, the amount of epiphytic fungi species of the organic grape was slightly higher than that of conventional grape; on the contrary, the amount of epiphytic bacteria species of the organic grape was lower. The number of epiphytic microorganisms of organic grape was more than that of conventional grape on all organs except leaf. The organic farming was shown to be more effective to keep the biodiversity of microbial communities in ecological environment.

Key words: red globe grape, organic food, epiphyte, food safety

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