食品科学 ›› 2011, Vol. 32 ›› Issue (3 ): 165-170.doi: 10.7506/spkx1002-6630-201103039

• 生物工程 • 上一篇    下一篇

利用来源于Paenibacillus macerans的α-CGTase突变体Y89D生产α-环糊精

王 宁1,2,吴 丹1,2,陈 晟1,2,陈 坚1,2,吴 敬1,2,*   

  1. 1.江南大学食品科学与技术国家重点实验室,江苏无锡 214122;
    2.江南大学生物工程学院,工业生物技术教育部重点实验室,江苏无锡 214122
  • 收稿日期:2010-04-30 修回日期:2011-01-11 出版日期:2011-02-15 发布日期:2011-01-13
  • 通讯作者: 吴敬 E-mail:jingwu80@hotmail.com
  • 基金资助:

    国家杰出青年基金项目(20625619);食品科学与技术国家重点实验室科研基金项目(SKLF-MB-200802);国家“973”项目(2007CB 714036)

Preparation of α-Cyclodextrin by Mutation Y89D of Cyclodextrin Glycosyltransferase from Paenibacillus macerans

WANG Ning,WU Dan,CHEN Sheng,CHEN Jian,WU Jing   

  1. 1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China ;2. Key Laboratory of
    Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China
  • Received:2010-04-30 Revised:2011-01-11 Online:2011-02-15 Published:2011-01-13

摘要: 对α- 环糊精葡萄糖基转移酶(α-CGTase)突变体Y89D 制备α- 环糊精的影响因素进行初步研究。其因素包括淀粉种类(马铃薯淀粉、玉米淀粉、木薯淀粉、可溶性淀粉)、加酶量、反应时间、pH 值、有机溶剂(乙醇、异丙醇、正丁醇、正癸醇)和温度。结果表明:选用5g/100mL 马铃薯淀粉、pH5.0、温度30℃、加酶量控制在 每克淀粉5U 左右,反应体系中加入体积分数5% 的正癸醇,反应6h 后,淀粉总转化率可达70%,其中α- 环糊精在产物中质量分数约为85%,转化产物中含有少量β- 环糊精(15%),而极少生成γ- 环糊精。因此,α-CGTase突变体Y89D 在制备α- 环糊精工艺中具有很好的工业化应用前景。

关键词: α-环糊精, α-环糊精葡萄糖基转移酶, 有机溶剂, 转化率

Abstract: The factors for preparingα-cyclodextrin by mutation Y89D of α-cyclodextrin glycosyltransferase (α-CGTase) were investigated. The factors include starch types (potato, corn, cassava and soluble starch), enzyme amount, reaction time, pH, organic solvents (ethanol, isopropanol, n-butanol and n-decanol) and temperature. Results indicated that the optimal conditions were 5 g/100 mL potato starch, 5 U/g CGTase, pH 5.0, 30 ℃, 5% n-decanol and reaction time of 6 h. Under the optimal conditions, 70% conversion rate of starch to cyclodextrins was achieved, which included 85% α-cyclodextrin, 15% β-cyclodextrin and trace γ -cyclodextrin. Therefore, the mutation Y89D of α -CGTase has promising industrial application prospect.

Key words: &alpha, -cyclodextrin, &alpha, -cyclodextrin glycosyltransferase, organic solvent, conversion rate

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