食品科学 ›› 2011, Vol. 32 ›› Issue (3 ): 171-175.doi: 10.7506/spkx1002-6630-201103040

• 生物工程 • 上一篇    下一篇

一株从泡菜中分离的产细菌素乳杆菌的鉴定及细菌素特性研究

张国强1 , 2,樊明涛2,师俊玲2,方江平1 ,*   

  1. 1.西藏农牧学院,西藏林芝 860000;2.西北农林科技大学食品科学与工程学院,陕西杨凌 712100
  • 收稿日期:2010-04-06 修回日期:2011-01-11 出版日期:2011-02-15 发布日期:2011-01-13
  • 通讯作者: 张国强 E-mail:guoqiangz2008@yahoo.com.cn
  • 基金资助:

    “十一五”国家科技支撑计划项目(N2006BAC01A04;2007BAC06B08)

Detection and Characterization of a Broad-Range Bacteriocin Produced by Lactobacillus brevis SD-22, Isolated from Traditional Chinese Pickles

ZHANG Guo-qiang,FAN Ming-tao,SHI Jun-ling,FANG Jiang-ping   

  1. 1. College of Agriculture and Husbandry, Tibet University, Linzhi 860000, China;
    2. College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China
  • Received:2010-04-06 Revised:2011-01-11 Online:2011-02-15 Published:2011-01-13
  • Contact: Zhang Guo-qiang E-mail:guoqiangz2008@yahoo.com.cn

摘要: 从泡菜中分离的329 株乳酸菌中,筛选出一株产细菌素的乳酸菌,编号为SD-22。经生理生化实验鉴定为短乳杆菌(Lactobacillus brevis)。在排除过氧化氢干扰和有机酸抑菌作用后,brevicin SD-22 对革兰氏阳性细菌和革兰氏阴性细菌具有较好的抑制作用,对部分真菌也有抑制作用,但对酵母菌和青霉无抑制作用,具有良好的热稳定性和pH 值稳定性,经胰蛋白酶、胃蛋白酶和蛋白酶K 处理后抑菌活性完全消失,对α- 淀粉酶不敏感。因此初步认为该菌株是一株产广谱细菌素的乳酸菌。

关键词: 乳杆菌, 细菌素, 特性

Abstract: Lactobacillus brevis SD-22 was selected from 329 lactic acid bacteria isolated from traditional Chinese pickles, The strain was found able to produce a bacteriocin strongly inhibited Staphylococcus aureus ATCC 63586 and Escherichia coli ATCC 24936. This bacteriocin, which was designated brevicin SD-22, was purified by ammonium sulphate precipitation for further studies. Brevicin SD-22 exhibited a wide range of antimicrobial activity, strong heat stability ( 121 ℃, 15 min) and pH stability (pH 3.0-8.0). Brevicin SD-22 was sensitive to protease but insensitive to α-amylase. Thus, Brevicin SD-22 has the potential to be applied in food preservation.

Key words: Lactobacillus brevis, bacteriocin, characterization

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