乳源β- 酪啡肽-7 对高血糖大鼠小肠黏膜氧化应激的影响

食品科学 ›› 2011, Vol. 32 ›› Issue (3 ): 208-211.

乳源β- 酪啡肽-7 对高血糖大鼠小肠黏膜氧化应激的影响

印虹，马畅，韩东宁，张源淑*

南京农业大学农业部生理生化重点开放实验室

收稿日期:2010-05-26 修回日期:2011-01-06 出版日期:2011-02-15 发布日期:2011-01-13
通讯作者: 张源淑 E-mail:zhangyuanshu@njau.edu.cn;zhangyuanshu@sina.com

Effects of β -CM-7 on the Oxidative Stress of Small Intestine Mucosa in Diabetic Rats

Received:2010-05-26 Revised:2011-01-06 Online:2011-02-15 Published:2011-01-13

摘要/Abstract

摘要： 为了探讨主要的乳源活性肽β- 酪啡肽-7 对高血糖大鼠小肠黏膜氧化应激损伤的保护作用。本研究选用SD雄性大鼠43 只，随机取35 只诱导高血糖模型，另8 只作为空白对照。造模成功大鼠24 只随机分为高、低剂量组和模型对照组。高、低剂量组每12h 灌胃β-CM-7 溶液1mL，高剂量组β-CM-7 浓度为7.5 × 10-6mol/L，低剂量组β-CM-7 浓度为7.5 × 10-7mol/L，模型对照组、空白对照组大鼠按等体积蒸馏水灌胃。连续灌胃30d 后，断颈处死，取血浆、小肠黏膜等进行小肠黏膜Na+-K+-ATP 酶、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性与丙二醛含量测定。结果表明：低剂量组能显著提高高血糖大鼠小肠黏膜Na+-K+-ATP 酶活力及CAT 活力(P ＜ 0.05)；高、低剂量组均能显著提高高血糖大鼠小肠黏膜SOD、GSH-Px 酶活力(P ＜ 0.05)；低剂量组能显著降低高血糖大鼠小肠黏膜MDA 含量。结论：β-CM-7 可以通过上调小肠黏膜SOD、GSH-Px 等抗氧化应激酶活性，缓解高血糖氧化应激产生的自由基对机体小肠黏膜的损伤。

关键词: 高血糖, 小肠黏膜, Na+-K+-ATP酶, 氧化应激

Abstract: The present study was aimed to investigate the possible protective effects of β-casomorphin -7 in small intestine mucosa in diabetic rats. A total of 43 male SD rats were used for this stud and were divided into two groups of control (n = 8) and hyperglycemia group (n = 35). The hyperglycemia rats (n = 24) were randomly divided into three groups: positive control rats, high dose β-CM-7 treated diabetic rats, and low dose β-CM-7 treated diabetic rats. On the last day of treatment, the animals were deprived of food overnight and sacrificed by cervical dislocation. Blood was collected at the presence of anticoagulant and the small intestine mucosa was obtained. The MDA level and the activity of Na+-K+-ATPase, SOD, GSH-Px, and CAT of small intestine mucosa were measured. The results showed after 30 days oral administration of β-casomorphin -7, the activity of Na+- K+-ATPase was markedly increased compared with positive control rats. Oral administration of high or low does of β-CM-7 to diabetic rats showed an increase in the activity of SOD, and GSH-Px, and low does also showed the reducing function on the elevated MDA level in diabetic rats. Thus the results of the present study suggest that β -casomorphin -7 can decrease the oxidative stress in small intestine mucosa in diabetic rats by elevating the activity of enzymatic antioxidants including SOD and GSH-Px.

Key words: hyperglycemia, small intestine mucosa, Na+-K+-ATPase, oxidative stress

中图分类号:

S858

印虹马畅韩东宁张源淑. 乳源β- 酪啡肽-7 对高血糖大鼠小肠黏膜氧化应激的影响[J]. 食品科学, 2011, 32(3 ): 208-211.

[1]	祝敏，靳林，赵浩安，王倩，张锦锦，杨二林，曹炜. 紫穗槐蜂蜜对小鼠急性酒精性胃溃疡的预防作用[J]. 食品科学, 2021, 42(9): 77-84.
[2]	杨成林，黄超，刘娟，黄卫梅，袁志航，易金娥，梁曾恩妮，邬静. 桦木酸对T-2毒素诱导小鼠肝损伤的保护作用[J]. 食品科学, 2021, 42(9): 107-114.
[3]	黄琳琳，张一敏，朱立贤，梁荣蓉，罗欣，成海建，毛衍伟. 蛋白质氧化和翻译后修饰对肉品质的影响及机制研究进展[J]. 食品科学, 2021, 42(9): 241-247.
[4]	杨忠敏，沈以红，黄先智，王祖文，丁晓雯. 桑叶生物碱对氧化应激小鼠糖脂代谢异常及肝损伤的改善作用[J]. 食品科学, 2021, 42(7): 156-161.
[5]	陈慧, YOSHINORI Katakura, 扈洪波, 尹淑涛, 赵冲. SIRT3多酚激活剂的筛选及其对UVB诱导HaCaT细胞衰老的修复作用[J]. 食品科学, 2021, 42(5): 115-121.
[6]	黄燕婷，梅丽华，潘海博，覃璐琪，饶川艳，聂梦琳，朱雯君，李全阳. 巴马长寿特征饮食模式对自然衰老小鼠的抗衰老效果[J]. 食品科学, 2021, 42(5): 137-144.
[7]	何萍萍，郑雅君，郑明静，姜泽东，杜希萍，朱艳冰，倪辉，李清彪. 红毛藻多糖对H2O2诱导的Caco-2细胞氧化损伤的保护作用[J]. 食品科学, 2021, 42(17): 113-120.
[8]	李西栋，孙绍霞，梁亚楠，石塔拉，宓伟. 山楂原花青素和VC联合通过Wnt/β-catenin通路减轻胰岛素抵抗大鼠肝脏氧化应激[J]. 食品科学, 2021, 42(17): 186-192.
[9]	杨诗怡，林巍，杨丽玉，左成，罗勤. CodY在单核细胞增生李斯特菌抗氧化胁迫中的作用[J]. 食品科学, 2021, 42(14): 46-53.
[10]	牙甫礼，XIN Yu，张春梅，陈彬林，李玮琪，马永洁. 姜黄素对H2O2诱导血小板凋亡的抑制作用及分子机制[J]. 食品科学, 2021, 42(13): 151-157.
[11]	胡博然，丁建才，曹杨，田颖，国凤华，袁静. (＋)-儿茶素与表没食子儿茶素没食子酸酯对乙醇诱导HepG2细胞脂代谢紊乱及氧化应激的影响[J]. 食品科学, 2021, 42(13): 114-120.
[12]	华梅，樊美玲，李志满，董丽娜，李珊珊，孙印石. 人参水溶性膳食纤维对大鼠糖脂代谢、氧化应激和肠道健康的影响[J]. 食品科学, 2021, 42(13): 127-135.
[13]	王新怡，张一敏，梁荣蓉，董鹏程，罗欣，毛衍伟. 过氧化物氧化还原酶6调控肉品嫩度的研究进展[J]. 食品科学, 2021, 42(11): 236-243.
[14]	刘子微，朱建津，陈巍文，李浙烽. 阿魏酸类似物对强的松诱导的小鼠氧化应激的影响[J]. 食品科学, 2021, 42(1): 163-171.
[15]	肖宝平，陈露，曾珺，刘静雯，李健，李桂玲. 姜黄素提高PC12细胞的抗氧化能力[J]. 食品科学, 2021, 42(1): 172-179.