食品科学 ›› 2011, Vol. 32 ›› Issue (3 ): 223-227.

• 营养卫生 • 上一篇    下一篇

花盖红菇菌丝体提取物的体外功效及组分分析

袁云辉1,张树斌1,姚钢乾2,赵俊霞3,王立安1 ,*   

  1. 1.河北师范大学生命科学学院 2.河北旅游职业学院生物工程系
    3.河北医科大学基础医学院
  • 收稿日期:2010-04-20 修回日期:2011-01-08 出版日期:2011-02-15 发布日期:2011-01-13
  • 通讯作者: 王立安 E-mail:wlian1965@126.com
  • 基金资助:

    河北省自然科学基金项目(C2008000155);河北省教育厅重点研究计划项目(ZH2007107)

Bioactivity and Chemical Components of Mycelial Extracts of Russula cyanoxantha

  • Received:2010-04-20 Revised:2011-01-08 Online:2011-02-15 Published:2011-01-13

摘要: 采用摇瓶发酵获得花盖红菇菌丝体,用石油醚、乙酸乙酯、乙醇和水提取菌丝体活性物质,得到不同的提取物。采用清除DPPH 自由基实验和抑制小鼠肝细胞脂质过氧化活性实验检测各提取物的抗氧化活性。结果显示:乙酸乙酯提取物和乙醇提取物均具有较高的抗氧化活性,其中以乙醇提取物活性最高。MTT 法检测各提取物对H446 肺癌细胞增殖的抑制作用,结果表明:石油醚提取物、乙酸乙酯提取物及乙醇提取物均有较高的抗肿瘤活性。采用色谱技术对乙醇提取物进行分离纯化,得到3 个单一组分,分别命名为组分Ⅰ、Ⅱ、Ⅲ;经结构鉴定组分Ⅰ为1- 氮杂[4,2,0]双环-4- 烯-8- 酮。

关键词: 花盖红菇, 菌丝体, 乙醇提取物, 抗氧化, 抗肿瘤

Abstract: Russula cyanoxantha was cultured using liquid fermentation medium. Mycelia of Russula cyanoxantha were subjected to extraction by petroleum ether (PE), ethyl acetate (EA) and ethanol, respectively. Antioxidant activities of different extraction fractions were evaluated by scavenging capacity on DPPH free radicals and inhibitory rate of lipid peroxidation. Results indicated that EA-soluble extract and ethanol-soluble extract had better antioxidant activities and the ethanol-soluble extract revealed the highest antioxidant capability. MTT assay indicated that PE, EA and ethanol extracts had obvious inhibition effects on the proliferation of H446 cancer cells, suggesting the anti-tumor activities of these extracts. The compositions of ethanol extract from mycelia of Russula cyanoxantha were analyzed. Three fractions were achieved by chromatographic separation as Fraction I, II and III, respectively. Fraction I was identified as 1-aza-bicyclo [4.2.0] oct-4-en-8-one by IR, FAB-MS, 1H NMR, 13C NMR, HSQC and HMBC methods.

Key words: Russula cyanoxantha, mycelia, ethanol extract, antioxidant activity, anti-tumor activity

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