食品科学 ›› 2011, Vol. 32 ›› Issue (5 ): 342-350.doi: 10.7506/spkx1002-6630-201105074

• 专题论述 • 上一篇    下一篇

表面等离子体共振技术在农药检测研究中的应用

林 钊,刘 霞*,李 迎   

  1. 湖南农业大学食品科技学院,食品科学与生物技术湖南省重点实验室
  • 收稿日期:2010-07-13 修回日期:2011-02-15 出版日期:2011-03-15 发布日期:2011-03-03
  • 通讯作者: 刘霞 E-mail:liuxia608@gmail.com
  • 基金资助:
    湖南省教育厅优秀青年项目(10B050);长沙市科技计划重点项目(K1005181-21);
    湖南农业大学引进人才基金项目(08YJ07);湖南省研究生创新基金项目(CX2010B281)

Recent Advances in Application of Surface Plasmon Resonance in Determining Pesticide Residues

LIN Zhao,LIU Xia*,LI Ying   

  1. Hunan Province Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural
    University, Changsha 410128, China
  • Received:2010-07-13 Revised:2011-02-15 Online:2011-03-15 Published:2011-03-03
  • Contact: LIU Xia*, E-mail:liuxia608@gmail.com

摘要: 表面等离子共振(SPR)技术具有灵敏度高、操作简单、能够实时监测反应的动态过程,只需对样品进行简单的预处理,无需进行标记,也可以无需纯化各种生物组分,耗样量少,检测时间短,已被广泛应用于各个研究领域。该技术不仅可以检测分析物,而且可以测定分子间相互作用的动力学常数。目前,SPR在检测食品和环境领域中的农药残留做了大量的研究工作。本文简单介绍SPR的基本原理以及SPR生物传感器的类型,重点综述SPR生物传感器应用于农药检测中传感芯片的识别分子种类,检测方法以及目前SPR检测农药的研究现状,最后对SPR生物传感器应用于农药检测领域的发展前景作出展望。

关键词: 表面等离子共振(SPR), 传感器, 农药, 检测

Abstract: Surface plasmon resonance (SPR) has been widely applied in various fields owing to its simplicity, low cost, no labeling, high selectivity, and real-time monitoring. SPR can be used not only for detecting analytes, but also determining kinetic parameters of molecular interactions. Interest in the development of SPR based immunosensors for the detection of low molecular weight pesticides in foods and environment has been rapidly increased over the last ten years. In this paper, the SPR principle and instrument types are briefly introduced. The category of recognized molecules immobilized on the SPR sensor surface, detection methods and their applications in pesticide detection are introduced emphatically. In addition, trends of SPR in the area of pesticide detection are also prospected.

Key words: surface plasmon resonance (SPR), sensor, pesticide, detection

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