食品科学 ›› 2011, Vol. 32 ›› Issue (5 ): 45-49.doi: 10.7506/spkx1002-6630-201105010

• 基础研究 • 上一篇    下一篇

柿子皮提取物的抗氧化活性

贾贵东,杨建雄*,王 芳,徐 乐,包 闽   

  1. 陕西师范大学生命科学学院
  • 收稿日期:2010-06-20 修回日期:2011-01-23 出版日期:2011-03-15 发布日期:2011-03-03
  • 通讯作者: 杨建雄 E-mail:jxyang@snnu.edu.cn
  • 基金资助:
    国家自然科学基金项目(20175012);陕西师范大学大学生开放性实验基金项目

Antioxidant Activity of Persimmon Peel Extracts

JIA Gui-dong,YANG Jian-xiong*,WANG Fang,XU Le,BAO Min   

  1. College of Life Science, Shaanxi Normal University, Xi’an 710062, China
  • Received:2010-06-20 Revised:2011-01-23 Online:2011-03-15 Published:2011-03-03
  • Contact: YANG Jian-xiong E-mail:jxyang@snnu.edu.cn

摘要: 为评价柿子皮提取物(persimmon peel extracts,PPE)的抗氧化活性,采用不同的抗氧化评价方法以及Fe2+-H2O2或Fe2+-VC诱导的离体鼠肝线粒体损伤模型。结果显示:PPE具有良好的总抗氧化活性、还原力、脂质过氧化抑制力以及自由基清除能力;清除DPPH自由基、超氧阴离子自由基(O2·)、羟自由基(·OH)的IC50值分别为57、137、425μg/mL;另外,PPE能显著地抑制线粒体脂质过氧化、肿胀、蛋白氧化和ATPase活性降低。说明PPE有明显的体外抗氧化活性,有可能作为一种天然抗氧化剂的新原料。

关键词: 柿子皮, 抗氧化, 自由基, 线粒体

Abstract: In order to evaluate the antioxidant activity of persimmon peel extracts (PPE) in vitro, different antioxidant evaluation assays and a mouse model of liver mitochondrial damage induced by Fe2+-H2O2 or Fe2+-VC in vitro were used. The results showed that PPE possessed excellent total antioxidant activity, reducing power, inhibitory activity against lipid peroxidation and radical scavenging activity. The IC50 values against DPPH, superoxide anion and hydroxyl radicals were 57, 137μg/mL and 425 μg/mL, respectively. In addition, PPE could significantly protect mitochondria from lipid peroxidation, swelling, protein oxidation and the reduction of ATPase activity in rats. In conclusion, the current study showed that PPE can resist oxidation obviously in vitro and be utilized as a new natural source of antioxidant.

Key words: persimmon peel, antioxidant, free radical, mitochondria

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