食品科学 ›› 2011, Vol. 32 ›› Issue (5 ): 79-82.doi: 10.7506/spkx1002-6630-201105017

• 基础研究 • 上一篇    下一篇

北五味子乙素清除自由基及体外抑菌作用的研究

李 斌,孟宪军*,薛 雪,吴 倩,李元甦,汪艳群   

  1. 沈阳农业大学食品学院
  • 收稿日期:2010-05-24 修回日期:2011-01-22 出版日期:2011-03-15 发布日期:2011-03-03
  • 通讯作者: 孟宪军 E-mail:mengxjsy@126.com
  • 基金资助:
    “十一五”国家科技支撑计划项目(2006BAI09B04);沈阳农业大学青年教师基金项目(2010)

Free Radical-scavenging and Antimicrobial Activities of Schisandrin B from Schisandra chinensis

LI Bin,MENG Xian-jun*,XUE Xue,WU Qian,LI Yuan-su,WANG Yan-qun   

  1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China
  • Received:2010-05-24 Revised:2011-01-22 Online:2011-03-15 Published:2011-03-03
  • Contact: MENG Xian-jun E-mail:mengxjsy@126.com

摘要: 对五味子乙素进行清除自由基及体外抑菌实验。结果表明:五味子乙素对自由基的清除效果明显,其中对羟自由基清除作用大于相同质量浓度的VC,其半数清除质量浓度为0.2mg/mL;对超氧阴离子自由基清除作用小于VC,其半数清除质量浓度为0.24mg/mL。五味子乙素提取液对金黄色葡萄球菌、白色念珠菌、沙门氏菌、大肠杆菌、枯草芽孢杆菌均有一定的抑制作用,其中对白色念珠菌和金黄色葡萄球菌的抑制作用最强,最低抑菌浓度为0.25mg/mL,对根霉、黑曲霉、南洋酵母无明显抑菌活性。

关键词: 北五味子, 乙素, 清除自由基, 抑菌

Abstract: Schisandrin B obtained from the fruits of Schisandra chinensis by supercritical carbon dioxide extraction and subsequent preparative HPLC separation was tested for its free radical-scavenging and antimicrobial activities in vitro. The results showed that Schisandra chinensis-derived schisandrin B had higher hydroxyl radical scavenging effect (IC50 = 0.2 mg/mL) but lower superoxide anion radical scavenging effect (IC50 = 0.24 mg/mL) when compared with vitamin C at the same concentrations. The compound could inhibit Staphylococcus aureus, Candida albicans, Salmonella, E.coli and Bacillus subtilis, especially against Candida albicans and Staphylococcus aureus with a minimum inhibitory concentration (MIC) of 0.25 mg/mL. However, no obvious inhibitory effect on Rhizopus, Aspergillus niger or Nanyang yeast was observed.

Key words: Schisandra chinensis (Turcz.) Baill, schisandrin B, free radical-scavenging activity, antimicrobial activity

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