食品科学 ›› 2011, Vol. 32 ›› Issue (5 ): 187-191.doi: 10.7506/spkx1002-6630-201105040

• 生物工程 • 上一篇    下一篇

发酵法生产灵芝-蕨渣菌质可溶性膳食纤维的工艺优化

余有贵1,2,李忠海1,黄国华2,李 金2,周军燕2   

  1. 1.中南林业科技大学食品科学与工程学院 2.邵阳学院生物与化学工程系
  • 收稿日期:2010-05-19 修回日期:2011-01-24 出版日期:2011-03-15 发布日期:2011-03-03
  • 通讯作者: 余有贵 E-mail:yufly225@yahoo.com.cn

Optimization of Solid-state Fermentation Conditions of Destarched Bracken Roots by Ganoderma lucidum for Production of Soluble Dietary Fiber

YU You-gui1,2,LI Zhong-hai1,HUANG Guo-hua2,LI Jin2,ZHOU Jun-yan2   

  1. 1. College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China;
    2. Department of Biological and Chemical Engineering,Shaoyang University, Shaoyang 422004, China
  • Received:2010-05-19 Revised:2011-01-24 Online:2011-03-15 Published:2011-03-03
  • Contact: YU You-gui E-mail:yufly225@yahoo.com.cn

摘要: 为了获得发酵法制备灵芝-蕨渣菌质可溶性膳食纤维的最适工艺参数,以基质蕨渣比例、基质含水量和培养温度为因子,以可溶性膳食纤维得率为响应值,采用响应面试验设计进行试验。结果表明:可溶性膳食纤维最适制备条件为基质蕨渣质量分数84%、基质含水量为63%、培养温度27℃。在此条件下,可溶性膳食纤维得率的预测值为13.034%,验证实验所得可溶性膳食纤维得率为12.885%。回归方程的预测值和实验值差异不显著,所得回归模型拟合情况良好,达到设计要求。在本实验优化的条件下,以发酵法制备灵芝-蕨渣菌质的可溶性膳食纤维得率(12.885%)显著高于蕨渣原料的可溶性膳食纤维得率(1.733%)。

关键词: 蕨渣, 灵芝, 发酵工艺, 固体菌质, 可溶性膳食纤维

Abstract: Destarched bracken roots were solid-state fermented with Ganoderma lucidum and the fermentation residue was harvested and submitted to enzymatic hydrolysis and subsequent absolute ethanol precipitation to obtain soluble dietary fiber (SDF). The amounts of destarch bracken roots and water in fermentation medium and culture temperature were identified as main factors that influence SDF yield, and optimized by response surface methodology combined with central composite design. The results showed that the optimum amounts of destarch bracken roots and water in fermentation medium and culture temperature were 84%, 63% and 27 ℃, respectively. Under these conditions, the predicted and experimental values of SDF yield were 13.034% and 12.885%, respectively. The fact that they were not significantly different demonstrated that the developed regression model could experimental data well and was qualified. Moreover, the experimental value of SDF yield (12.885%) was significantly higher than the value of 1.733% obtained without Ganoderma lucidum fermentation before enzymatic hydrolysis.

Key words: destarched bracken roots, Ganoderma lucidum, fermentation process, solid-sate, soluble dietary fiber

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