关键词: 寿光鸡, 聚合酶链式反应-单链构象多态(PCR-SSCP), CAPN1基因, 肉质性状

Abstract: Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method was used to determine the polymorphism of exon 4 of CAPN1 gene in 147 Shouguang chicken, and analyze the correlation between CAPN1 gene and meat quality traits in Shouguang chicken. Three genotypes were identified as AA, AB and BB with the genotype frequencies of 0.286, 0.510 and 0.204, respectively. Statistical analysis indicated that type BB Shouguang chicken had significantly higher breast muscle pHu value than type AA (P＜0.05). However, type AA Shouguang chicken had significantly higher content of intramuscular fat than type BB (P＜0.05).

Key words: Shouguang chicken, PCR-SSCP, CAPN1 gene, meat quality traits