蜂胶和铁补充对运动大鼠血液铁状态的影响及蜂胶的抗氧化保护作用

doi:10.7506/spkx1002-6630-201105052

食品科学 ›› 2011, Vol. 32 ›› Issue (5 ): 244-248.doi: 10.7506/spkx1002-6630-201105052

蜂胶和铁补充对运动大鼠血液铁状态的影响及蜂胶的抗氧化保护作用

车力龙¹，刘军²，刘显东²，沈敏杰²，顾晓明²，解洋²，许化溪¹，肖德生¹

1.江苏大学基础医学与医学技术学院 2.江苏大学临床医学院

收稿日期:2010-06-17 修回日期:2011-01-19 出版日期:2011-03-15 发布日期:2011-03-03
通讯作者: 车力龙 E-mail:cll8880@163.com
基金资助:
国家自然科学基金项目(30270639)；江苏大学大学生科技创新项目(1201390005；1201390009)

Effect of Propolis and Iron Supplements on Iron Status in Blood of Exercise Rats and Antioxidant Function of Propolis

CHE Li-long1，LIU Jun2，LIU Xian-dong2，SHEN Min-jie2，GU Xiao-ming2，XIE Yang2，XU Hua-xi1，XIAO De-sheng1

1. School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, China；
2. School of Clinical Medicine, Jiangsu University, Zhenjiang 212001, China

Received:2010-06-17 Revised:2011-01-19 Online:2011-03-15 Published:2011-03-03
Contact: CHE Li-long1 E-mail:cll8880@163.com

摘要/Abstract

摘要： 目的：观察蜂胶和铁补充对运动大鼠血液铁状态指标和血浆自由基代谢的影响，研究蜂胶对运动和/或铁补充过程中产生的氧化损伤的抗氧化保护作用。方法：40只3月龄Sprague-Dawley雄性大鼠，被随机分为5组：对照组(C组)、运动组(E组)、运动补铁组(EI组)、运动补蜂胶组(EP组)和运动补铁同时补蜂胶组(EIP组)，每组8只。6周递增负荷跑台训练后检测各组大鼠红细胞指标(红细胞数(RBC)、血红蛋白(Hb)、红细胞比容(Hct))和血浆铁状态指标(血浆铁(PI)和总铁结合力(TIBC))；并检测血浆MDA和SOD值。结果：E组红细胞和铁状态指标显著低于C组，MDA值显著高于C组，SOD值显著低于C组。与E组比较，EI组、EP组和EIP组的红细胞、铁状态指标均显著升高；EI组MDA值显著高于E组，SOD值显著低于E组；EP组和EIP组MDA值显著低于E组，SOD值显著高于E组。EIP组MDA值显著低于EI组，SOD值显著高于EI组，但与EP组无显著差异。结论：虽然铁补充能改善运动大鼠贫血和低铁状态，但增加了大鼠氧化应激风险。蜂胶能有效提高机体清除自由基能力，减少脂质过氧化，增强抗氧化酶活性；对改善运动大鼠贫血和低铁状态也有一定作用。

关键词: 蜂胶, 铁补充, 运动大鼠, 铁状态, 抗氧化

Abstract: Objective: To explore the effects of propolis and iron supplements on iron status and free radical content in blood of exercise rats, and to investigate the antioxidative protection of propolis on oxidative damage derived from exercise and/or iron supplement. Methods: Forty Sprague-Dawley male rats aged 3 months were randomly divided into 5 groups of 8 each: control group (C group), exercise group (E group), exercise plus iron supplement group (EI group), exercise plus propolis supplement group (EP group) and exercise plus supplement with iron and propolis group (EIP group). The rats were subjected to treadmill training with an incremental loading for 6 weeks. Red blood cell indices including red blood cell count (RBC) and hemoglobin (Hb) and hematocrit (Hct), plasma iron status including plasma iron (PI) and total iron binding capacity, and serum MDA and SOD contents of rats were measured. Results: Compared with C group, a significantly lower level of red blood cell indices, iron status and SOD, and an obvious higher level of MDA were observed in E group. Compared with E group, a significantly higher level of red blood cell indices and iron status was observed in rats from EI, EP and EIP groups; meanwhile, MDA content in rats from EI group was higher than that in E group; SOD content in rats from EI group was lower than that in E group. MDA content of rats in EP and EIP groups was lower than that in E group and SOD content of rats in EP and EIP groups was higher than that in E group. MDA content in rats from EIP group was lower than that in EI group and SOD content in rats from EIP group were higher than that in EI group. No significant difference between EP and EIP groups was also observed. Conclusion: Iron supplement can increase the risk of oxidative stress in spite of improving anemia symptom and lower iron status of exercise rats. Propolis can effectively improve the ability of organism to scavenge free radicals, reduce lipid peroxidation, and enhance antioxidant function. Therefore, propolis can improve the exercise-induced or iron-deficient anemia.

Key words: propolis, iron supplement, exercise rats, iron status, antioxidation

中图分类号:

车力龙1，刘军2，刘显东2，沈敏杰2，顾晓明2，解洋2，许化溪1，肖德生1. 蜂胶和铁补充对运动大鼠血液铁状态的影响及蜂胶的抗氧化保护作用[J]. 食品科学, 2011, 32(5 ): 244-248.

CHE Li-long1，LIU Jun2，LIU Xian-dong2，SHEN Min-jie2，GU Xiao-ming2，XIE Yang2，XU Hua-xi1，XIAO De-sheng1. Effect of Propolis and Iron Supplements on Iron Status in Blood of Exercise Rats and Antioxidant Function of Propolis[J]. FOOD SCIENCE, 2011, 32(5 ): 244-248.

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蜂胶和铁补充对运动大鼠血液铁状态的影响及蜂胶的抗氧化保护作用

Effect of Propolis and Iron Supplements on Iron Status in Blood of Exercise Rats and Antioxidant Function of Propolis

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