琐琐葡萄总黄酮在体外对乙型肝炎病毒的影响

doi:10.7506/spkx1002-6630-201105058

食品科学 ›› 2011, Vol. 32 ›› Issue (5 ): 270-272.doi: 10.7506/spkx1002-6630-201105058

琐琐葡萄总黄酮在体外对乙型肝炎病毒的影响

刘涛¹,马龙¹,赵军²,李海波³

1. 新疆医科大学公共卫生学院卫生毒理学教研室
2.新疆维吾尔自治区药物研究所 3.江苏省南通市疾病预防控制中心

收稿日期:2010-06-18 修回日期:2011-01-21 出版日期:2011-03-15 发布日期:2011-03-03
通讯作者: 马龙 E-mail:xjmult@163.com
基金资助:
国家自然科学基金项目(30660157)

Inhibitory Effects of Total Flavones from Vitis vinifer L. on Hepatitis B Virus in vitro

LIU Tao1，MA Long1,*，ZHAO Jun2，LI Hai-bo3

1. Department of Toxicology, College of Public Health, Xinjiang Medical University, Urumqi 830011, China；
2. Institute of Materia Medica of Xinjiang, Urumqi 830004, China；
3. Nantong Center for Disease Control and Prevention, Nantong 226006, China

Received:2010-06-18 Revised:2011-01-21 Online:2011-03-15 Published:2011-03-03
Contact: MA Long1 E-mail:xjmult@163.com

摘要/Abstract

摘要： 目的：考察琐琐葡萄总黄酮体外抗乙型肝炎病毒(HBV)的效果。方法：用HBV DNA转染人肝癌细胞所得的HepG 2.2.15细胞株为模型，ELISA法测定用药后8d细胞培养上清液中HBsAg和HBeAg含量，计算琐琐葡萄总黄酮对HBsAg和HBeAg分泌的抑制率，荧光定量PCR法测定HBV DNA的变化，MTT比色法检测细胞毒性。结果：琐琐葡萄总黄酮在12.5～100μg/mL范围内，均能不同程度地减少细胞培养上清液中HBsAg和HBeAg的含量，且细胞毒性小，半数中毒剂量(TC50)为284.91μg/mL，对HBsAg半数有效剂量(IC50)为327.56μg/mL、对HBeAg为215.34μg/mL，对HBsAg治疗指数(TI)为0.87、对HBeAg治疗指数为1.32；总黄酮质量浓度100μg/mL时对HBV DNA的抑制率为47.63%。结论：琐琐葡萄总黄酮具有一定的体外抗HBV作用。

关键词: 琐琐葡萄, 黄酮, 乙型肝炎病毒, 细胞培养

Abstract: Objective: To investigate the inhibitory effects of total flavones from Vitis vinifer L. on hepatitis B virus (HBV) replication and their toxicity in cultured human hepatocellular liver carcinoma cell line HepG 2.2.15 transfected with HBV DNA. Methods: After 8 days of treatment with total flavones from Vitis vinifer L., the contents of HBsAg and HBeAg in the cell culture supernatant of HepG 2.2.15 were measured by ELISA to calculate the inhibitory rates against the secretion of HBsAg and HBeAg, the change in HBV DNA was monitored by real-time PCR, and cytotoxicity test was performed using MTT assay. Results: Addition of total flavones from Vitis vinifer L. in a dosage range of 12.5 to 100μg/mL could reduce the contents of HBsAg and HBeAg in the cell culture supernatant of HepG 2.2.15 to different extents. Little cytotoxic effect was observed with a median toxic concentration (TC50) of 284.91 μg/mL. The median effective doses (IC50) on HBsAg and HBeAg were 327.56 μg/mL and 215.34 μg/mL, and the therapeutic index for HBsAg and HbeAg were 0.87 and 1.32, respectively. Total flavones from Vitis vinifer L. at 100 μg/mL could inhibit HBV DNA by 47.63%. Conclusion: Total flavones from Vitis vinifer L. have inhibitory effects on HBV in vitro.

Key words: Vitis vinifer L, flavones, hepatitis B virus, cell culture

中图分类号:

R965

刘涛1，马龙1,*，赵军2，李海波3. 琐琐葡萄总黄酮在体外对乙型肝炎病毒的影响[J]. 食品科学, 2011, 32(5 ): 270-272.

LIU Tao1，MA Long1,*，ZHAO Jun2，LI Hai-bo3. Inhibitory Effects of Total Flavones from Vitis vinifer L. on Hepatitis B Virus in vitro[J]. FOOD SCIENCE, 2011, 32(5 ): 270-272.

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琐琐葡萄总黄酮在体外对乙型肝炎病毒的影响

Inhibitory Effects of Total Flavones from Vitis vinifer L. on Hepatitis B Virus in vitro

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