大孔树脂吸附纯化青钱柳叶三萜化合物

doi:10.7506/spkx1002-6630-201106014

食品科学 ›› 2011, Vol. 32 ›› Issue (6 ): 61-65.doi: 10.7506/spkx1002-6630-201106014

大孔树脂吸附纯化青钱柳叶三萜化合物

尹忠平¹,上官新晨^1.2,张月红²,黎冬明²,吴少福²,陈继光²

1.南昌大学食品科学与技术国家重点实验室 2.江西农业大学植物资源开发与利用研究室

收稿日期:2010-05-19 修回日期:2011-01-14 出版日期:2011-03-25 发布日期:2011-03-03
通讯作者: 尹忠平 E-mail:yinzp2008@yahoo.com.cn
基金资助:
江西省科技支撑计划项目(2009BSB09100)；江西省自然科学基金项目(2007GZN0214)

Purification of Triterpenoids from Cyclocarya paliurus Leaves by Macro-porous Resin Adsorption

YIN Zhong-ping1,2，SHANGGUAN Xin-chen1,2,*，ZHANG Yue-hong2，LI Dong-ming2，WU Shao-fu2，CHEN Ji-guang2

1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China；
2. Laboratory of Plant Resources Exploitation and Utilization, Jiangxi Agricultural University, Nanchang 330045, China

Received:2010-05-19 Revised:2011-01-14 Online:2011-03-25 Published:2011-03-03
Contact: YIN Zhong-ping1 E-mail:yinzp2008@yahoo.com.cn

摘要/Abstract

摘要： 为得到纯度较高的青钱柳叶三萜化合物，采用大孔树脂吸附法对青钱柳叶三萜粗提物进行纯化研究。静态吸附实验结果表明，AB-8树脂对青钱柳叶三萜化合物吸附、解吸性能较好，比饱和吸附量为77.49mg/g，解吸率为85.44%，总回收率可达72.01%。AB-8树脂动态吸附-解吸实验表明：上柱流速增大，吸附率呈降低趋势；随着上柱液质量浓度增大，吸附率先升后降；95%乙醇洗脱效果较好，10BV为合理用量；随着洗脱流速增大，洗脱液峰浓度降低，洗脱率减小，综合考虑以1.5mL/min较为适宜。经AB-8树脂纯化后，三萜类物质纯度可达44.30%。

关键词: 青钱柳, 三萜化合物, 大孔树脂, 纯化

Abstract: In order to obtain triterpenoids with high purity from Cyclocarya paliurus leaves, macro-porous resin was used for purification. Results indicated that macro-porous resin revealed a static absorption mode. AB-8 resin had good adsorption capability on triterpenoids from Cyclocarya paliurus leaves, and its adsorption capacity, desorption rate and total recovery rate were 77.49 mg/g, 85.44% and 72.01%, respectively. Dynamic adsorption and desorption experiments of AB-8 resin suggested that the increasing flow rate of sample solution could result in the decrease of adsorption rate, and the increasing sample amount in resin could result in a pattern of initial increase and final decline for adsorption rate. A better elution efficiency was observed at the concentration of 95% ethanol, and 10 BV was reasonable dosage. Both peak concentration and desorption rate were declined with the increasing flow rate of eluent. The best flow rate was 1.5 mL/min. The purity of triterpenoids purified by AB-8 macro-porous resin was 44.30%.

Key words: Cyclocarya paliurus, total triterpenoids, macro-porous resin, purification

中图分类号:

TQ028.15

尹忠平1,2，上官新晨1,2,*，张月红2，黎冬明2，吴少福2，陈继光2. 大孔树脂吸附纯化青钱柳叶三萜化合物[J]. 食品科学, 2011, 32(6 ): 61-65.

YIN Zhong-ping1,2，SHANGGUAN Xin-chen1,2,*，ZHANG Yue-hong2，LI Dong-ming2，WU Shao-fu2，CHEN Ji-guang2. Purification of Triterpenoids from Cyclocarya paliurus Leaves by Macro-porous Resin Adsorption[J]. FOOD SCIENCE, 2011, 32(6 ): 61-65.

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大孔树脂吸附纯化青钱柳叶三萜化合物

Purification of Triterpenoids from Cyclocarya paliurus Leaves by Macro-porous Resin Adsorption

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