食品科学 ›› 2011, Vol. 32 ›› Issue (6 ): 120-123.doi: 10.7506/spkx1002-6630-201106026

• 工艺技术 • 上一篇    下一篇

新疆大蓟总黄酮的超声提取及抗氧化性研究

史礼貌,解成喜*   

  1. 新疆大学理化测试中心
  • 收稿日期:2010-06-03 修回日期:2011-01-21 出版日期:2011-03-25 发布日期:2011-03-03
  • 通讯作者: 解成喜 E-mail:xiecx@xju.edu.cn

Ultrasonic Extraction and Antioxidant Activity of Total Flavonoids from Cirsium japonicum

SHI Li-mao,XIE Cheng-xi*   

  1. Physical and Chemical Analysis Center, Xinjiang University, Urumqi 830046, China
  • Received:2010-06-03 Revised:2011-01-21 Online:2011-03-25 Published:2011-03-03
  • Contact: XIE Cheng-xi* E-mail:xiecx@xju.edu.cn

摘要: 为研究新疆大蓟总黄酮的超声提取方法及其抗氧化活性,采用乙醇超声提取新疆大蓟中的总黄酮,分光光度法测定新疆大蓟中的总黄酮含量,研究新疆大蓟中黄酮类化合物对超氧阴离子自由基、羟自由基的清除作用。结果表明,新疆大蓟黄酮超声提取的最佳条件为乙醇体积分数75%、料液比1:30(g/mL)、超声时间45min,该条件下总黄酮提取率为0.289%,新疆大蓟中黄酮类化合物对清除自由基有明显的作用,且黄酮类化合物的添加量在实验范围内与其抗氧化性呈正相关。

关键词: 分光光度法, 新疆大蓟, 超声法, 总黄酮, 抗氧化

Abstract: Objective: To explore the optimal ultrasonic extraction processing and antioxidant activity of total flavonoids from Cirsium japonicum. Methods: The total flavonoids in Cirsium japonicum were extracted by ultrasound-assisted alcohol extraction method and the content of total flavonoids was determined by UV spectrophotometry. The antioxidant activity of total flavonoids from Cirsium japonicum was evaluated by the scavenging capability for superoxide anion and hydroxyl free radicals. Results: The optimal extraction conditions for total flavonoids from Cirsium japonicum were ethanol concentration of 75%; material/liquid ratio of 1:30 and ultrasonic treatment time of 45 min. Under the optimal extraction conditions, the extraction rate of total flavonoids was 0.289%. The total flavonoids from Cirsium japonicum exhibited an obvious scavenging effect on free radicals. The antioxidant activity of total flavonoids was a dose-dependent in the certain concentration range.

Key words: spectrophotometry, Cirsium japonicum, ultrasonic extraction, total flavonoids, antioxidant

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