食品科学 ›› 2011, Vol. 32 ›› Issue (6 ): 155-158.doi: 10.7506/spkx1002-6630-201106035

• 分析检测 • 上一篇    下一篇

衍生分光光度法测定麦麸粗提液中阿魏酸的含量

杨 雪,姚艳艳,张志清*   

  1. 四川农业大学食品学院
  • 收稿日期:2010-05-07 修回日期:2011-01-07 出版日期:2011-03-25 发布日期:2011-03-03
  • 通讯作者: 张志清 E-mail:zqzhang721@163.com
  • 基金资助:
    教育部大学生创新性实验项目(091062627);四川省人事厅留学人员择优项目(川人函【2008】488 号);
    四川省教育厅重点项目(09ZA081)

Determination of Ferulic Acid in Wheat Bran by Derivatization Combined with Spectrophotometry

YANG Xue,YAO Yan-yan,ZHANG Zhi-qing*   

  1. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
  • Received:2010-05-07 Revised:2011-01-07 Online:2011-03-25 Published:2011-03-03
  • Contact: Zhi-Qing ZHANG E-mail:zqzhang721@163.com

摘要: 目的:建立衍生分光光度法测定麦麸粗提液中阿魏酸的含量。方法:以0.6g/100mL FeCl3-0.9g/100mL K3[Fe(CN)6](1:0.9)混合溶液为显色剂,并以显色剂为空白,在720nm波长处测定吸光度。结果:阿魏酸在2.36~14.16μg/mL范围内,吸光度与阿魏酸的质量浓度呈良好的线性关系(r=0.9996),最小检出量为0.022μg/mL,平均加标回收率为103.1%。结论:本检测方法操作简便、准确、回收率高,适用于麦麸粗提液中阿魏酸含量的测定。

关键词: 麦麸, 阿魏酸, 衍生分光光度法

Abstract: Objective: To establish a determination method of ferulic acid in wheat bran by derivatization combined with spectrophotometry. Methods: Ferulic acid was analyzed spectrophotometrically at 720 nm. The color-developing agent was the mixture of 0.6 g/100 mL FeCl3 and 0.9 g/100 mL K3Fe(CN)6] (1:0.9). Results: A good linear relationship in the concentration range of 2.36-14.16μg/mL for ferulic acid (r = 0.9996) was observed, and the detection limit of this developed method was 0.022μg/mL. The average recovery rate of this method was 103.1%. Conclusion: This developed method is characteristics of simple operation, accurate results and high average recovery rate, which is suitable for the determination of ferulic acid in wheat bran.

Key words: wheat bran, ferulic acid, derived spectrophotometry

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