食品科学 ›› 2011, Vol. 32 ›› Issue (6 ): 230-232.doi: 10.7506/spkx1002-6630-201106053

• 分析检测 • 上一篇    下一篇

高效液相色谱法测定水果中吡虫啉农药残留量

何进林1,张志华2   

  1. 1.广西出入境检验检疫局贺州办事处 2.山西出入境检验检疫局技术中心
  • 收稿日期:2010-05-10 修回日期:2011-01-11 出版日期:2011-03-25 发布日期:2011-03-03

Determination of Imidaclprid Residue in Fruits by HPLC

HE Jin-lin1,ZHANG Zhi-hua2   

  1. 1. Hezhou Dumex, Guangxi Entry-exit Inspection and Quarantine Bureau, Hezhou 542800, China;
    2. Technology Center, Shanxi Entry-exit Inspection and Quarantine Bureau, Taiyuan 030024, China
  • Received:2010-05-10 Revised:2011-01-11 Online:2011-03-25 Published:2011-03-03

摘要: 建立高效液相色谱法测定水果中的吡虫啉残留量的检测方法。采用改进的QuEChERS方法,乙腈匀浆提取,提取液加C18和PSA填料净化,液相色谱法检测。结果表明:方法的定量检测限为0.05mg/kg,标准曲线线性相关系数0.9988;当添加水平在0.05~0.20mg/kg范围内时,回收率均可在92%~102%之间,相对标准偏差在5%~11%之间。本方法是一种快速、准确、灵敏度高的同时检测水果中吡虫啉残留的检测方法。

关键词: 水果, 液相色谱法, 吡虫啉

Abstract: A high performance liquid chromatography (HPLC) method was developed to determine imidaclprid residue in fruits. Samples were extracted by the improved QuEChERS method using acetonitrile as the solvent and purified on C18/PSA column. The detection limit of this developed HPLC method was 0.05 mg/kg and the linear correlation coefficient of standard curve was 0.9988. Recovery rates for imidaclprid in spiked samples at 0.05, 0.1 mg/kg and 0.20 mg/kg was in the range of 92%-102% with a relative standard deviation varying from 5% to 11%. Therefore, this developed method is characteristics of rapid determination, high accuracy and sensitivity so that it is suitable for the detection of imidaclprid residue in fruits.

Key words: fruits, HPLC, imidaclprid

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