食品科学 ›› 2011, Vol. 32 ›› Issue (6 ): 233-236.doi: 10.7506/spkx1002-6630-201106054

• 分析检测 • 上一篇    下一篇

快速分离液相-四级杆串联飞行时间质谱分析大豆中的异黄酮成分

郑振佳1.2,初玉圣3,迟炳海3,王晓1.2   

  1. 1.山东农业大学食品科学与工程学院 2.山东省分析测试中心 3.山东龙大食品集团有限公司
  • 收稿日期:2010-04-30 修回日期:2011-02-10 出版日期:2011-03-25 发布日期:2011-03-03
  • 通讯作者: 王晓 E-mail:wxjn1998@126.com
  • 基金资助:
    国家自然科学基金项目(20872083);山东省科技攻关项目(2009GG2001021-16);
    作物生物学国家重点实验室开放课题(20082KF05)

Analysis of Isoflavonoids in Soybean by Rapid Resolution Liquid Chromatography-Quatrupole-Time of  Flight Mass Spectrometry

ZHENG Zhen-jia1,2,CHU Yu-sheng3,CHI Bing-hai3,WANG Xiao1,2,*   

  1. 1. College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China;
    2. Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, China;
    3. Shandong Longda Foodstuff Group Co. Ltd., Yantai 265231, China
  • Received:2010-04-30 Revised:2011-02-10 Online:2011-03-25 Published:2011-03-03
  • Contact: WANG Xiao E-mail:wxjn1998@126.com

摘要: 建立快速分离液相-四级杆飞行时间串联质谱(RRLC-Q-TOF)联用技术分析大豆中的异黄酮的方法。大豆样品经甲醇超声辅助提取后,选用Welch Materials C18柱,以乙腈-水(含0.1%甲酸)为流动相进行梯度洗脱。结果表明在正离子模式下,经快速分离液相-四级杆飞行时间串联质谱分离并检测出9种异黄酮成分。RRLC-Q-TOF联用技术可以快速准确鉴定样品中的异黄酮成分。

关键词: 快速分离液相/四级杆串联飞行时间质谱, 大豆, 异黄酮

Abstract: A novel method for the analysis of isoflavonoids in soybean was established by rapid resolution liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (RRLC-Q-TOF-MS). Samples were extracted with methanol under ultrasound assistance, and then a Welch Materials C18 column was applied in the RRLC separation using acetonitrile and water (0.1% formic acid) as the mobile phase. Elutes were detected by Q-TOF to obtain MS spectra with a positive ion mode. Nine isoflavonoids were identified. Therefore, this method can be used to rapidly determine isoflavonoids of soybean.

Key words: RRLC-Q-TOF, soybean, isoflavonoids

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