食品科学 ›› 2010, Vol. 31 ›› Issue (13 ): 139-142.doi: 10.7506/spkx1002-6630-201013033

• 基础研究 • 上一篇    下一篇

黑木耳提取物对大肠杆菌生物膜形成的抑制作用

李 斌,董明盛*   

  1. 南京农业大学食品科技学院
  • 收稿日期:2010-04-06 出版日期:2010-07-01 发布日期:2010-12-29
  • 通讯作者: 董明盛 E-mail:dongms@njau.edu.cn
  • 基金资助:

    国家自然科学基金项目(30700627);江苏省农业科技自主创新项目(CX(08)113)

Inhibition Effect of Auricularia auricula Extract on Biofilm Formation of Escherichia coli

LI Bin DONG Ming-sheng*   

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2010-04-06 Online:2010-07-01 Published:2010-12-29
  • Contact: DONG Ming-sheng E-mail:dongms@njau.edu.cn

摘要:

采用卡尔加里生物膜装置和菌落计数法研究黑木耳粗提物对大肠杆菌Escherichia.coli 8099生物膜形成的影响,同时利用光学显微镜和激光共聚焦显微镜观察黑木耳粗提物对大肠杆菌生物膜形态结构的影响。结果表明:黑木耳粗提物能有效抑制大肠杆菌生物膜的形成,其质量浓度为0.3g/mL时抑制率可以达到73.00%。

关键词: 黑木耳, 大肠杆菌, 生物膜, 抑制

Abstract:

The inhibition effect of Auricularia auricula extract on the biofilm formation of Escherichia coli 8099 was investigated using Calgary biofilm device and colony count method. Meanwhile, the morphology and structure of E. coli biofilm were also examined using optical microscope and confocal laser scanning microscope after treatment of Auricularia auricula extract. The results indicated 0.3 g/mL of Auricularia auricula extract obviously inhibited the biofilm formation of E. coli and the inhibition rate reached up to 73.0%.

Key words: Auricularia auricula, Escherichia coli, biofilm, inhibition

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