食品科学 ›› 2010, Vol. 31 ›› Issue (9 ): 202-205.doi: 10.7506/spkx1002-6630-201009045

• 生物工程 • 上一篇    下一篇

胆固醇氧化酶高产菌株的筛选及鉴定

蔡 丹,张娜娜,刘景圣*   

  1. 吉林农业大学食品科学与工程学院
  • 收稿日期:2009-12-30 出版日期:2010-05-01 发布日期:2010-12-29
  • 通讯作者: 刘景圣 E-mail:liujs1007@vip.sina.com.cn
  • 基金资助:

    吉林农业大学青年启动基金项目(2007027)

Screening and Identification of Cholesterol Oxidase-producing Strains with High Activity

CAI Dan,ZHANG Na-na,LIU Jing-sheng*   

  1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China
  • Received:2009-12-30 Online:2010-05-01 Published:2010-12-29
  • Contact: LIU Jing-sheng E-mail:liujs1007@vip.sina.com.cn

摘要:

将从不同地点采集到的9 个样品进行分离筛选,从中分离到23 株以胆固醇为唯一碳源的菌株,确定其中1 株胞外胆固醇氧化酶高产菌株A3,其胆固醇氧化酶的活力为525U/L。经过形态学和生理生化实验初步鉴定,该菌株属于短杆菌属(Brevibacterium sp.)。

关键词: 胆固醇氧化酶, 菌株, 筛选

Abstract:

Twenty-three strains using cholesterol as the sole carbon source were isolated from nine samples collected from different locations. One strain with high yield of extracellular cholesterol oxidase was screened and named as strain A3. The highest activity of cholesterol oxidase was 525 U/L. The strain A3 was identified as Brevibacterium sp. through analyzing its morphology, physiological and biochemical characteristics.

Key words: cholesterol oxidase, strain, screening

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