树脂法优化大豆皂甙的纯化条件及其结构表征

doi:10.7506/spkx1002-6300-201004026

食品科学 ›› 2010, Vol. 31 ›› Issue (4 ): 116-119.doi: 10.7506/spkx1002-6300-201004026

树脂法优化大豆皂甙的纯化条件及其结构表征

关海宁¹，刁小琴¹，史波林^{2 ,*} ，马松艳¹

1.绥化学院生物与食品工程系 2.上海大学通信与信息工程学院

收稿日期:2009-10-22 出版日期:2010-02-15 发布日期:2010-12-29
通讯作者: 史波林 E-mail:shibl@cnis.gov.cn
基金资助:
绥化学院科研创新团队资助项目(CX2008002)

Macroporous Resin Purification and Structural Characterization of Soybean Saponins

GUAN Hai-ning1，DIAO Xiao-qin1，SHI Bo-lin2,*，MA Song-yan1

1. Department of Biology and Food Engineering, Suihua University, Suihua 152061, China；
2. School of Communication and Information Engineering, Shanghai University, Shanghai 200072, China

Received:2009-10-22 Online:2010-02-15 Published:2010-12-29
Contact: SHI Bo-lin E-mail:shibl@cnis.gov.cn

摘要/Abstract

摘要：

研究大孔吸附树脂对低温脱脂豆粕中大豆皂甙的吸附分离特性。选择4 种大孔吸附树脂，通过比较对大豆皂甙的静态与动态的吸附和解吸特征，筛选出最佳吸附剂，并对其吸附动力学特征及解吸条件进行研究。结果表明：AB-8 型树脂吸附与洗脱的效果最佳；优化出最佳工艺参数为以2BV 去离子水除杂后的体积分数70%乙醇溶液为洗脱溶媒，以0.5BV/h 流速进行洗脱，收集洗脱液经减压蒸馏及真空冷冻干燥，得皂甙纯品，测定产品纯度为87.07%，得率1.19%。并对冻干纯品进行傅里叶红外光谱表征，鉴定出产品结构与已知大豆皂甙结构一致且纯度可靠。

关键词: 大豆皂甙, 大孔吸附树脂, 纯化, 结构表征

Abstract:

To screen an optimal adsorbent for the purification of soybean saponins from low-temperature defatted soybean meal, four types of macroporous resin were compared for the differences in dynamic and static adsorption and desorption performances. AB-8-type macroporous resin was shown to have optimal adsorption and desorption capacities towards soybean saponins. The optimal desorption parameters for the purification of soybean saponins using AB-8-type macroporous resin were investigated, and the structure of purified soybean saponins was characterized by infrared (IR) spectroscopy. Results showed that the optimal elution of soybean saponins from AB-8-type macroporous resin was achieved by washing the resin with 2 BV deionized water followed by eluting with 70% ethanol at a flow rate of 0.5 BV/h. The eluate was concentrated by reduced pressure distillation and vacuum freeze dried and purified soybean saponins with 87.07% purity and 1.19% yield were obtained. In addition, the structure of purified product was confirmed by IR spectroscopy to be consistent with the known structure of soybean saponins.

Key words: soybean saponins, macroporous resin, purification, structural characterization

中图分类号:

Q946.83

关海宁1，刁小琴1，史波林2,*,马松艳1. 树脂法优化大豆皂甙的纯化条件及其结构表征[J]. 食品科学, 2010, 31(4 ): 116-119.

GUAN Hai-ning1，DIAO Xiao-qin1，SHI Bo-lin2,*，MA Song-yan1. Macroporous Resin Purification and Structural Characterization of Soybean Saponins[J]. FOOD SCIENCE, 2010, 31(4 ): 116-119.

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树脂法优化大豆皂甙的纯化条件及其结构表征

Macroporous Resin Purification and Structural Characterization of Soybean Saponins

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