食品科学 ›› 2009, Vol. 30 ›› Issue (24 ): 51-55.doi: 10.7506/spkx1002-6630-200924008

• 工艺技术 • 上一篇    下一篇

Alcalase 蛋白酶制备鲤鱼鱼鳞胶原多肽

韩 冰1,石彦国1,张根生1,杨 慧1,李彦宏2   

  1. 1.哈尔滨商业大学食品工程学院 2.山东商务职业学院食品工程系
  • 收稿日期:2009-08-16 出版日期:2009-12-15 发布日期:2010-12-29
  • 通讯作者: 韩 冰1 E-mail:hb790118@163.com

Preparation of Hydrolyzed Collagen from Cyprinoid Scales

HAN Bing1,SHI Yan-guo1,ZHANG Gen-sheng1,YANG Hui1,LI Yan-hong2   

  1. 1. College of Food Engineering, Harbin University of Commerce, Harbin 150076, China;
    2. Department of Food Engineering, Shandong Business Institute, Yantai 264670, Ch
  • Received:2009-08-16 Online:2009-12-15 Published:2010-12-29
  • Contact: HAN Bing1, E-mail:hb790118@163.com

摘要:

以实验室自制的脱钙鲤鱼鱼鳞为原料,采用Alcalase 蛋白酶对其进行水解,制备鱼鳞胶原多肽。在此过程中,以蛋白质提取率和羟脯氨酸含量为指标,采用单因素及正交试验方法优化酶解条件,考察预处理温度、料液比、酶解温度、加酶量、酶解时间等对蛋白质提取率的影响。结果表明,鱼鳞胶原蛋白预处理条件为121℃加热处理10min、酶解温度60℃、底物质量分数12%、加酶量0.06AU/g、酶解时间3h。在最佳工艺条件下,蛋白质提取率可达96.24%。

关键词: 鲤鱼, 鱼鳞, 胶原多肽, 酶解

Abstract:

Decalcified cyprinoid scales were used as materials to prepare hydrolyzed collagen through alcalase hydrolysis. Pretreatment temperature, substrate concentration, hydrolysis temperature, enzyme concentration and hydrolysis time were optimized through evaluating extraction rate of collagen and content of hydroxyproline by single-factor and orthogonal experiments. Results indicated that optimal hydrolysis conditions were 60 ℃ of hydrolysis temperature, 12% substrate amount, 0.06 AU/g of enzyme, and 3 h of hydrolysis time. The extraction rate of collagen from cyprinoid scales was 96.24% under this optimal condition.

Key words: cyprinoid, scale, hydrolyzed collagen, hydrolysis

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