食品科学 ›› 2009, Vol. 30 ›› Issue (24 ): 114-117.doi: 10.7506/spkx1002-6630-200924022

• 工艺技术 • 上一篇    下一篇

超滤法分离梅花鹿胎盘寡肽的工艺研究

皮钰珍,曹晓宇,王淑琴   

  1. 沈阳农业大学食品学院
  • 收稿日期:2009-07-11 出版日期:2009-12-15 发布日期:2010-12-29
  • 通讯作者: 皮钰珍, E-mail:Yuzhen_pi@sina.com
  • 基金资助:

    辽宁省博士启动基金项目(20081068)

Separation of Oligopeptides from Deer Placenta via Ultrafiltration

PI Yu-zhen,CAO Xiao-yu,WANG Shu-qin   

  1. College of Food Science, Shenyang Agricultural University, Shenyang 110161, Chi
  • Received:2009-07-11 Online:2009-12-15 Published:2010-12-29
  • Contact: PI Yu-zhen, E-mail:Yuzhen_pi@sina.com

摘要:

采用超滤法对梅花鹿胎盘中活性寡肽进行分离,考察料液质量分数、压力、温度、流速、浓缩倍数及操作时间因素对分离效果的影响,确定最佳分离工艺条件为料液质量分数1.5%、压力0.06MPa、温度30℃、流速2.0ml/s、浓缩倍数2.5 倍、操作时间3min,分离后的样品中蛋白含量为56.22%。膜污染后用0.2% NaOH 溶液浸泡2h,循环1h 后,用盐酸浸泡1h,循环0.5h,再用水洗至中性,膜通量可得到较好的恢复,流量恢复率为0.834。

关键词: 梅花鹿胎盘寡肽, 超滤, 膜污染

Abstract:

Active oligopeptides are the most effective ingredients in deer placenta. The oligopeptides was separated through ultrafiltration from deer placenta. The major factors such as material-liquid ratio, pressure, temperature, flow rate, condensation folds and operation time were investigated to evaluate the effect on separation efficiency. The optimal separation condition was 1.5% of material-liquid ratio, 0.06 MPa of pressure, 30 ℃ of temperature, 2.0 ml/s of flow rate, 2.5 folds for condensation degree and 3 min of operation time. Protein content in separated samples was 56.22% under this optimal condition. The contaminated membrane was cleaned using 0.2% NaOH for 2 h of soaking and 1 h of circulation first, then using HCl for 1 h of soaking and 0.5 h of circulation, and finally using water washing until neutrality. An excellent recovery rate for membrane flux was achieved and WFR value was 0.834.

Key words: deer placenta oligopeptide, ultrafiltration, membrane contamination

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