食品科学 ›› 2009, Vol. 30 ›› Issue (24 ): 213-216.doi: 10.7506/spkx1002-6630-200924045

• 工艺技术 • 上一篇    下一篇

超临界CO2 流体色谱法分离丁香酚与异丁香酚

谢建春1,韩蕙阑1,程 劼2,肖叶兰1,孙宝国1,郑福平1   

  1. 1.北京工商大学化学与环境工程学院 2.中国农业科学院农业质量标准与检测技术研究所
  • 收稿日期:2009-09-22 出版日期:2009-12-15 发布日期:2010-12-29
  • 通讯作者: 谢建春1, E-mail:xjchun@thth.btbu.edu.cn
  • 基金资助:

    北京市教委科技发展计划重点项目

Separation of Eugenol and Isoeugenol by Supercritical CO2 Fluid Chromatography

XIE Jian-chun1,HAN Hui-lan1,CHENG Jie2,XIAO Ye-lan1,SUN Bao-guo1,ZHENG Fu-ping1   

  1. 1. School of Chemical and Environmental Engineering, Beijing Technology and Business University, Beijing 100048, China;
    2. Institute of Quality Standard and Testing Technologies for Agro-products, Chinese Accademy of Agricultural Sciences,
    Beijing 100081, China
  • Received:2009-09-22 Online:2009-12-15 Published:2010-12-29
  • Contact: XIE Jian-chun1, E-mail:xjchun@thth.btbu.edu.cn

摘要:

用超临界CO2 流体色谱分离丁香酚与异丁香酚两个双键位置异构体。以分离度为指标,考察改性剂、柱压、柱温3 个因素对分离的影响,优化使用硅胶色谱柱和氰基色谱柱分离丁香酚与异丁香酚的色谱条件。结果表明,使用氰基柱的分离效果更好,异丁香酚的顺、反异构体也得到分离。在紫外检测波长283nm、流动相流速1.5ml/min 的条件下,进一步考察进样量对分离的影响,最终确定分离丁香酚、异丁香酚的色谱条件为氰基柱(250mm × 4.6mm,5μm),改性剂为体积分数8% 的甲醇、柱温40℃、柱压8MPa、进样质量体积10μl、样品质量浓度43.7mg/ml。此时分离度2.41,分离时间大约为7min。

关键词: 超临界CO2 流体色谱, 丁香酚, 异丁香酚, 分离

Abstract:

Supercritical CO2 fluid chromatography (SFC) was used to separate isomers, eugenol and isoeugenol in this paper. Effects of modifier, column pressure and column temperature on separation efficiency was evaluated by resolution factor under optimal silica column or cyano column. Results indicated cyano column provided better separation efficiency due to successful separation of trans- and cis-isomers of isoeugenol. The optimal separation condition for eugenol and isoeugenol was cyano column (250 mm × 4.6 mm, 5μm), UV detection wavelength at 283 nm, 8% modifier in methanol as mobile phase, 1.5 ml/min of flow rate for mobile phase, 40 ℃ of column temperature, 8 MPa column pressure, 10 μl of sample loading amount, and 43.7 mg/ml sample. The resolution factor was 2.41 with 7 min of running time under this optimal separation condition.

Key words: supercritical CO2 fluid chromatography, eugenol, isoeugenol, separation

中图分类号: