食品科学 ›› 2009, Vol. 30 ›› Issue (23 ): 343-346.doi: 10.7506/spkx1002-6300-200923078

• 生物工程 • 上一篇    下一篇

发酵法分离提纯大豆糖蜜中低聚糖的研究

崔希庆1,2,刘 畅1,2,董银卯2,何聪芬2,王昌涛2,*   

  1. 1.东北农业大学生命科学学院 2.北京工商大学 北京市植物资源研究开发重点实验室
  • 收稿日期:2009-09-05 出版日期:2009-12-01 发布日期:2010-12-29
  • 通讯作者: 王昌涛 E-mail:wangct@th.btbu.edu.cn
  • 基金资助:

    国家“863”计划项目(2007AA100404)

Isolation and Purification of Oligosaccharides from Soybean Molasses through Fermentation

CUI Xi-qing1,2,LIU Chang1,2,DONG Yin-mao2,HE Cong-fen2,WANG Chang-tao2,*   

  1. 1.Collge of Life Science, Northeast Agricultural University, Harbin 150030, China; 2. Beijing Key Laboratory of Plant Resources
    Research and Development, Beijing Technology and Business University, Beijing 100048, China
  • Received:2009-09-05 Online:2009-12-01 Published:2010-12-29
  • Contact: WANG Chang-tao E-mail:wangct@th.btbu.edu.cn

摘要:

对3 种酵母菌,1 种乳酸菌,1 种曲霉进行筛选实验,选出了消耗蔗糖最多,水苏糖、棉子糖保留率最高的1 株酵母菌。经单因素试验证明,其发酵大豆糖蜜的最佳条件为:发酵初始pH 值为5.0,接种量为2%(V/V),大豆糖蜜稀释8 倍,不添加任何生长因子,28℃、180r/min 的条件下发酵 12h。大豆糖蜜发酵液中各糖分的保留率为:蔗糖8.76%、棉子糖99.61%、水苏糖95.72%。

关键词: 低聚糖, 大豆糖蜜, 发酵, 保留率

Abstract:

One strain of yeast that has the function of high consumption to sucrose, and high retention rate to stachyose and raffinose was screened from three stains of yeast, one strain of Lactobacillus, one strain of Aspergillus. The optimal condition for the fermentation of soybean molasses was initial pH 5, 2% inoculation amount, 1:8 of dilution for soybean molasses, 180 r/min shaking and fermentation at 28 ℃ for 12 h. The retention rates in hydrolysates of soybean molasses were 8.76% for sucrose, 99.61% for raffinose and 95.72% for stachyose under this optimal fermentation condition.

Key words: oligosaccharide, soybean molasses, fermentation, retention rate

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