食品科学 ›› 2009, Vol. 30 ›› Issue (22 ): 40-43.doi: 10.7506/spkx1002-6300-200922005

• 工艺技术 • 上一篇    下一篇

一步法分离鸡蛋清中三种主要过敏原的研究

麻小娟1 ,2,习斌蓉3,陈红兵1 ,4,高金燕2 ,*   

  1. 1.南昌大学 食品科学与技术国家重点实验室 2.南昌大学生命科学与食品工程学院食品系 3.江西省妇幼保健院 4.南昌大学中德联合研究院
  • 收稿日期:2009-07-05 出版日期:2009-11-15 发布日期:2010-12-29
  • 通讯作者: 高金燕2 ,* E-mail:gjy1967@yahoo.com.cn
  • 基金资助:

    江西省自然科学基金项目(2008GZN0040);南昌大学食品科学与技术国家重点实验室自由探索项目(SKLF-TS-200820);江西省研究生创新专项资金项目

Simultaneous Separation of Three Major Allergens in Hen’s Egg White

MA Xiao-juan1,2,XI Bin-rong3,CHEN Hong-bing1,4,GAO Jin-yan2,*   

  1. (1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;2. Department of Food
    Science, School of Life Sciences and Food Engineering, Nanchang University, Nanchang 330047, China;3. Jiangxi Maternal and Child
    Health Hospital, Nanchang 330006, China ;4. Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China)
  • Received:2009-07-05 Online:2009-11-15 Published:2010-12-29
  • Contact: GAO Jin-yan2,* E-mail:gjy1967@yahoo.com.cn

摘要:

为快速高效分离鸡蛋中主要过敏原开展鸡蛋过敏的研究,本实验采用DEAE-Sepharose FF 色谱柱一步分离鸡蛋中的三种主要过敏原卵白蛋白、卵转铁蛋白和溶菌酶,通过SDS-PAGE 比对,纯度均高于相应标准品纯度,得率分别为87.66%、55.87% 和92.80%。本实验操作简便、设备简单、分离效率高,是一种分离鸡蛋过敏原的好方法。

关键词: 卵白蛋白, 卵转铁蛋白, 溶菌酶, 鸡蛋过敏

Abstract:

Hen's egg is one of the most common causes of food allergy. In order to obtain egg white allergens as a material for further studies, three major allergens, i.e., ovalbumin, ovotransferrin and lysozyme, were separated by DEAE-Sepharose FF chromatography, with recovery rates of 87.66%, 55.87 % and 92.80% and purities of higher than 90%, respectively.

Key words: ovalbumin, ovotransferrin, lysozyme, egg allergy

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