食品科学 ›› 2009, Vol. 30 ›› Issue (22 ): 77-80.doi: 10.7506/spkx1002-6300-200922014

• 工艺技术 • 上一篇    下一篇

阴香果实花色苷粗提物树脂纯化研究

黄思梅,张 镜*   

  1. 嘉应学院生命科学学院
  • 收稿日期:2009-06-23 出版日期:2009-11-15 发布日期:2010-12-29
  • 通讯作者: 张 镜* E-mail:zhangcqf@jyu.edu.cn
  • 基金资助:

    梅州市科技计划资助项目(2006A11)

Purification of Crude Anthocyanins from Cinnamoumum burmanu Fruits Using Macroporous Resins

HUANG Si-mei,ZHANG Jing*   

  1. College of Life Science, Jiaying University, Meizhou 514015, China
  • Received:2009-06-23 Online:2009-11-15 Published:2010-12-29
  • Contact: ZHANG Jing* E-mail:zhangcqf@jyu.edu.cn

摘要:

阴香花色苷粗提物石油醚除脂后,以大孔吸附树脂DA201、DM301、DS401、D101 和 DM-18 进行了纯化技术的研究。静态吸附实验结果表明:DM-18 对阴香花色苷的吸附力最强,吸附量57.93mg/g,静态吸附平衡时间120min,80% 乙醇溶液的解吸率88.47%。DM-18 吸附花色苷动态解吸参数正交试验结果是:70% 乙醇最适洗脱剂、流速0.75BV/h 及pH3.0。

关键词: 阴香, 花色苷, 大孔吸附树脂, 纯化

Abstract:

This study was conducted to develop a macroporous resin adsorption technique for the purification of crude anthocyanins from C. burmannii fruits. Following petroleum ether defatting, the crude anthocyanins were subjected to purification using different types of macroporous resins, such as DA201, DM301, DS401, D101 and DM-18. Static adsorption assays showed that DM-18 had the highest adsorption capacity towards anthocyanins of up to 57.93 mg/g with 120 min equilibrium adsorption time and 88.47% of anthocyanins were desorbed using 80% ethanol solution. Orthogonal array optimization suggested that the optimal conditions for desorption of anthocyanins from DM-18 were using 70% ethanol solution as desorption agent, flow rate 0.75 BV/h and pH 3.0.

Key words: C. burmannii, anthocyanins, macroporous resin, purification

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