食品科学 ›› 2009, Vol. 30 ›› Issue (22 ): 165-167.doi: 10.7506/spkx1002-6300-200922036

• 工艺技术 • 上一篇    下一篇

红景天中高纯度红景天苷制备工艺

戴传云,刘春玲,徐玉琼,任凌燕   

  1. 重庆科技学院生物系
  • 收稿日期:2009-06-03 出版日期:2009-11-15 发布日期:2010-12-29
  • 通讯作者: 戴传云 E-mail:cydai@126.com
  • 基金资助:

    重庆市教委科学技术基金项目(20070410029)

Preparation of High Purity Salidroside from Rhodida Rosea

DAI Chuan-yun,LIU Chun-ling,XU Yu-qiong,REN Ling-yan   

  1. (Department of Biology, Chongqing University of Science and Technology, Chongqing 401331, China)
  • Received:2009-06-03 Online:2009-11-15 Published:2010-12-29
  • Contact: DAI Chuan-yun, E-mail:cydai@126.com

摘要:

研究从红景天粗提物中分离纯化高纯度红景天苷的制备方法。红景天提取物经75% 乙醇提取,乙酸铅沉淀和水饱和正丁醇萃取等三步预处理,可使红景天苷富集,红景天苷含量达43.80%,然后将预处理后得到红景天苷粗品分别用大孔吸附树脂D101 和AB-8 以及硅胶进行柱层析,采用TLC 法和RRLC法分别进行红景天苷的定性和定量分析;结果表明经AB-8 柱层析后,依次用水和5% 乙醇洗脱,得到的红景天苷含量大于90%。该方法工艺安全简单、成本低,非常适合工业化生产。

关键词: 红景天苷, 分离提纯, 柱层析

Abstract:

A preparation method of high purity Salidroside from Rhodida rosea was investigated in this paper. The crude extract of Rhodida rosea was subjected to 75% ethanoll distilment, acetic plumbum precipitation and n-butanol extraction and the content of salidroside was 43.80%. The product was further adsorbed by macroporous resin (D101 and AB-8) and silica gel and qualitative and quantitative analysis were performed by TLC and RRLC. It was showed that the product after column chromatogram of AB-8 and elution by water and 5% ethanol, salidroside with high purity over 90% was obtained. This method was proved to the most effecient for preparation of salidroside from Rhodida rosea in large-scale.

Key words: salidroside, separation and purification, column chromatogram

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