食品科学 ›› 2009, Vol. 30 ›› Issue (22 ): 298-300.doi: 10.7506/spkx1002-6300-200922070

• 分析检测 • 上一篇    下一篇

槟榔油的分离及GC-MS 分析

张海德,黄玉林,韩 林   

  1. 海南大学食品学院
  • 收稿日期:2009-06-01 出版日期:2009-11-15 发布日期:2010-12-29
  • 通讯作者: 张海德 E-mail:zhanghaide@163.com
  • 基金资助:

    “十一五”国家科技支撑计划项目(2007BAD76B03)

Isolation of Betel Nut Oil for GC-MS Analysis

ZHANG Hai-de,HUANG Yu-lin,HAN Lin   

  1. College of Food Science, Hainan University, Haikou 570228, China
  • Received:2009-06-01 Online:2009-11-15 Published:2010-12-29
  • Contact: ZHANG Hai-de E-mail:zhanghaide@163.com

摘要:

采用硅胶柱层析、气相色谱- 质谱(GC-MS)联用方法对槟榔油进行分离纯化和成分分析,确定以正已烷-丙酮(8:2,V/V)体系作为槟榔油分离的洗脱液。分离纯化后的槟榔油经GC-MS 测定共有18 种脂肪酸,其中含量较高的有十四烷酸(肉豆蔻酸)26.08%、十八碳烯酸(油酸)24.20%、十八碳二烯酸(亚油酸)22.70%、十六烷酸(棕榈酸)14.09% 和十二烷酸(月桂酸)7.87%。可见,槟榔油可开发为具有调节血脂、延缓衰老的保健食品。

关键词: 槟榔油, 分离, GC-MS

Abstract:

A method based on silca column chromatograph purification and identification by GC-MS has been developed for the isolation of betel nut oil. The crude extract of betel nut oil dissolved in Petroleum ether was separated by TLC and eluted with cyclohexane-acetone (8:2, V/V) as mobile phase. Results showed that the purified betel nut oil comprised 18 types of fatty acids, among which fourteen acid myristic acid accounted for 26.08%, octadecenoic acid (oleic acid) for 24.20%, eighteen carbon acid (linoleic acid) for 22.70%, hexadecanoic acid (palmitic acid) for 14.09%, and dodecanoic acid (lauric acid) 7.87%. Due to diversity of unsaturated fatty acid, betel nut oil has a great potential to develop for health food with function of regulating blood fat and delaying senility.

Key words: betel nut oil, separating, GC-MS

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