食品科学 ›› 2009, Vol. 30 ›› Issue (19 ): 70-73.doi: 10.7506/spkx1002-6630-200919013

• 基础研究 • 上一篇    下一篇

纤维素酶对油菜蜂花粉细胞壁通透性改善的研究

张红城1,2,董 捷1,2,*,任向楠1,2,齐 萱1,2   

  1. 1.中国农业科学院蜜蜂研究所 2.农业部国家蜂产品加工专业分中心
  • 收稿日期:2009-06-21 出版日期:2009-10-01 发布日期:2010-12-29
  • 通讯作者: 董 捷1,2,*, E-mail:jiedon@126.com
  • 基金资助:

    农业部公益性行业(农业)科研专项经费项目(nyhyzx07-041);农业部基本科研业务费专项课题(200703);中国农业科学院蜜蜂研究所研发基金项目(200702)

Effect of Cellulase on Cell Wall Permeability of Rape Bee Pollen

ZHANG Hong-cheng1,2,DONG Jie1,2,*,REN Xiang-nan1,2,QI Xuan1,2   

  1. (1. Institute of Apicultural Research, Chinese Academy of Agricultural Science, Beijing 100093, China;
    2. National Research Center of Bee Product Processing, Ministry of Agriculture, Beijing 100093, China)
  • Received:2009-06-21 Online:2009-10-01 Published:2010-12-29
  • Contact: DONG Jie1,2,*, E-mail:jiedon@126.com

摘要:

研究纤维素酶对油菜蜂花粉细胞通透性的改善情况,结果表明:用纤维素酶酶解后,油菜蜂花粉液的蛋白溶解度、分散指数、可溶性糖含量均有所提高;pH6 为纤维素酶酶解的最适pH 值。

关键词: 蜂花粉, 纤维素酶, 酶解, 通透性

Abstract:

In order to investigate the effect of cellulase on cell wall permeability of rape bee pollen, cellulase digestion of rape bee pollen was conducted. Protein solubility, distribution coefficient, amount of soluble carbonhydrates in rape bee pollen exhibited an obvious increase following cellulase digestion. The optimal pH value for cellulase digestion was determined to be 6.

Key words: rape bee pollen, cellulase, emzymolysis, permeability

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