食品科学 ›› 2009, Vol. 30 ›› Issue (19 ): 125-127.doi: 10.7506/spkx1002-6630-200919027

• 基础研究 • 上一篇    下一篇

玉米芯中低聚木糖的定性定量研究

张培刚1,张静文2,张凤清2,徐 萍2,任保国1   

  1. 1.吉林工程技术师范学院 2.长春工业大学化学与生命科学学院
  • 收稿日期:2009-07-12 出版日期:2009-10-01 发布日期:2010-12-29
  • 通讯作者: 张培刚1, E-mail:swspx@126.com
  • 基金资助:

    吉林省教育厅基金资助项目(2007 第415 号)

Investigations of Xylooligosaccharides in Corncob

ZHANG Pei-gang1,ZHANG Jing-wen2,ZHANG Feng-qing2,XU Ping2,REN Bao-guo1   

  1. 1. Jilin Teacher's Institute of Engineering and Technology, Changchun 130052, China;
    2. School of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, China
  • Received:2009-07-12 Online:2009-10-01 Published:2010-12-29
  • Contact: ZHANG Pei-gang1, E-mail:swspx@126.com

摘要:

利用传统的水提醇沉法从玉米芯提取可溶性糖,通过部分酸水解、葡聚糖凝胶SephadexG-25 柱层析、薄层层析(TLC)、高效液相色谱(HPLC)等对玉米芯中低聚木糖的组成及含量进行研究。结果表明:玉米芯中低聚木糖种类以木二糖和木三糖为主,提取纯化得木二糖的纯度可达95.63%,得率为6.84%;木三糖的含量则较低,仅为0.22%。

关键词: 玉米芯, 低聚木糖, 木二糖, 木三糖, 组成, 含量

Abstract:

Water-soluble crude polysaccharides obtained from corncob through water extraction and ethanol precipitation were partially hydrolyzed by trifluoroacetic acid. The crude polysaccharides and their hydrolysates were separately purified (fractionated) by Sephadex G-25 chromatography to be fractions A and B and they were then analyzed by thin-layer chromatography (TLC) and high-pressure liquid chromatography (HPLC). Xylobiose and xylotriose were major components of xylooligosaccharides from corncob. Fraction B mainly consisted of xylobiose with a yield of 6.84% in chromatographic purification and the xylobiose content was as high as 95.63%. However, the xylotriose content was only 0.22%.

Key words: corncob, xylooligosaccharides, xylobiose, xylotriose, component, content

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