食品科学 ›› 2009, Vol. 30 ›› Issue (19 ): 294-296.doi: 10.7506/spkx1002-6630-200919069

• 营养卫生 • 上一篇    下一篇

丹酚酸B 对衰老小鼠学习、记忆的影响及作用机制研究

高 辉,何 玲,李春艳,吕江明   

  1. 吉首大学医学院药理教研室
  • 收稿日期:2009-06-14 出版日期:2009-10-01 发布日期:2010-12-29
  • 通讯作者: 高 辉, E-mail:gaohui9512@163.com
  • 基金资助:

    吉首大学医学院课题资助项目

Effects and Mechanisms of Salvianolic Acid B on Learning Capability and Memory in Aging Mice

GAO Hui,HE Ling,LI Chun-yan,LU Jiang-ming   

  1. Department of Pharmacology, Medical College of Jishou University, Jishou 416000, China
  • Received:2009-06-14 Online:2009-10-01 Published:2010-12-29
  • Contact: GAO Hui, E-mail:gaohui9512@163.com

摘要:

目的:研究丹酚酸B 的抗衰老作用及作用机制。方法:将30 只2 月龄小鼠随机分为青年组、模型组及丹酚酸B 组,皮下注射D- 半乳糖造成衰老模型,同时丹酚酸B 组每日腹腔注射丹酚酸B 22.5mg/kg,6 周后进行跳台和水迷宫实验,并处死小鼠检查脑组织SOD、MDA 及线粒体DNA。结果:丹酚酸B 组小鼠跳台和水迷宫实验成绩均明显优于模型组,脑组织SOD 活力提高、MDA 的含量降低,线粒体DNA 的量显著降低。结论:丹酚酸B 具有抗衰老作用,推测可能是通过抗氧化及影响线粒体DNA 而发挥作用的。

关键词: 丹酚酸B, 抗衰老, SOD, MDA, mtDNA

Abstract:

Totally 30 mice were randomly divided into young group, aging model group and salvianolic acid B (Sal B) treatment group. Aging model was successfully established by subcutaneous injection of D-galactose. Mice from Sal B treatment group were administered with a dose of 22.5 mg/kg through intraperitoneal injection. Morris water maze test and stand-jumping test were conducted at 6 weeks post-injection. The mice were scarified to determine superoxide dismutase (SOD) activity, malondialdehyde (MDA) and mitochondrial DNA (mtDNA) levels in brain tissue. Sal B treatment group exhibited better performance than aging model group on Morris water maze test and stand-jumping test. Moreover, Sal B treatment significantly reduced cerebral mtDNA and MDA levels and improved cerebral SOD activity in aging mice. Therefore, Sal B has the function to anti-aging due to the promotion of anti-oxidation and reduction of cerebral mtDNA level.

Key words: Sal B, anti-aging, SOD, MDA, mtDNA

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