食品科学 ›› 2009, Vol. 30 ›› Issue (17 ): 88-90.doi: 10.7506/spkx1002-6630-200917019

• 基础研究 • 上一篇    下一篇

富锌水溶性莼菜蛋白ZnP6B-G75-WP Ⅱ的分离纯化与组成分析

周毅峰1,唐巧玉2,周大寨2,吴永尧3   

  1. 1.湖北民族学院生物科学与技术学院,湖北恩施 445000;2.生物资源保护与利用湖北省重点实验室,湖北恩施
    445000;3.湖南农业大学生化与发酵工程实验室,湖南长沙 410128
  • 收稿日期:2009-06-17 出版日期:2009-09-01 发布日期:2014-04-14
  • 基金资助:

    生物资源保护与利用湖北省重点实验室(湖北民族学院)开放基金项目(200725);湖北省自然科学基金项目(2003ADA127)

Separation, Purification and Amino Acid Composition Analysis of Zinc-rich Water-soluble Protein ZnP6B-G75-WPⅡfrom Water Shield Leaves

ZHOU Yi-feng1,TANG Qiao-yu2,ZHOU Da-zhai2,WU Yong-yao3   

  1. 1. School of Biological Science and Technology, Hubei University for Nationalities, Enshi 445000, China;
    2. Key Laboratory for Protection and Utilization of Biological Resources of Hubei Province, Enshi 445000, China ;
    3. Laboratory of Biochemical and Fermentation Engineering, Hunan Agricultural University, Changsha 410128, China
  • Received:2009-06-17 Online:2009-09-01 Published:2014-04-14

摘要:

通过凝胶过滤层析法对富锌水溶性莼菜蛋白质组分WPⅡ进行分离纯化,利用SDS- 聚丙烯酰胺凝胶电泳法进行检测,并采用反相高效液相色谱(RT-HPLC)法对其氨基酸组成情况进行分析。结果发现:分离所得富锌莼菜水溶性蛋白组分ZnP6B-G75-WP Ⅱ由同一亚基组成,锌元素的相对含量为8.44mg/kg;ZnP6B-G75-WP Ⅱ由12 种氨基酸组成,其中甘氨酸含量很高,并含有锌结合相关的组氨酸、半胱氨酸、硒代半胱氨酸、精氨酸、赖氨酸等氨基酸,并在莼菜蛋白中发现含硒代半胱氨酸的蛋白质。

关键词: 莼菜, 锌, 水溶性蛋白, 分离纯化

Abstract:

A zinc rich water-soluble protein fraction named ZnP6B-G75-WPⅡwas separated from a zinc-rich water-soluble protein WP Ⅱ extracted from water shield leaves by Sephadex G-75 column chromatography and purified by Sepharose 6B column chromatography. The purity and the amino acid composition of ZnP6B-G75-WPⅡwere analyzed by SDS-PAEG and RT-HPLC, respectively. SDS analysis indicated that the molecule of ZnP6B- G75-WPⅡ was composed by a single subunit. The relative content of zinc was 8.44 mg/kg. ZnP6B- G75-WP Ⅱ was composed of 12 amino acids including glycin of high content and others such as histidine, cysteine, selenium-cysteine, arginine etc. which are related with zinc combination and selenium-cysteine was found to be contained in the protein.

Key words: water shield, zinc, water-soluble protein, separation and purification

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