关键词: 菜籽粕, 蛋白酶, 亚硫酸钠, 水解度

Abstract:

Peptides were prepared by hydrolysis of rapeseed meal by papain, neutrase and flavourzyme, respectively. The effects of sodium sulfite at low concentration on proteolysis rate of rapeseed meal were investigated. The hydrolysis rates of rapeseed protein by the three enzymes were increased by the addition of sodium sulfite to different extents. After the 60 min hydrolysis, the degrees of hydrolysis of rapeseed protein by papain, neutrase and flavourzyme with the addition of sodium sulfite were 2.5, 2.9 and 1.7 times of those in the absence of sodium sulfite, respectively. Size exclusion chromatographic analysis showed that the molecular weights of the rapeseed protein hydrolysates with the addition of sodium sulfite were lower than those without sodium sulfite.

Key words: rapeseed meal, protease, sodium sulfite, degree of hydrolysis