食品科学 ›› 2009, Vol. 30 ›› Issue (17 ): 239-243.doi: 10.7506/spkx1002-6630-200917055

• 生物工程 • 上一篇    下一篇

不同载体固定化GOD-GAL 双酶乳糖生物传感器核微孔膜的研究

宋海琼,庞广昌* ,李家鹏   

  1. 天津市食品生物技术重点实验室,天津商业大学生物技术与食品科学学院
  • 收稿日期:2009-04-12 出版日期:2009-09-01 发布日期:2014-04-14
  • 通讯作者: 庞广昌* E-mail:pgc@tjcu.edu.cn
  • 基金资助:

    国家科技攻关计划课题(2006BAD04A00)

Bi-enzyme Biosensor for Lactose Detection Based on Immobilized Glucose Oxidase and β-galactosidase onto Different Carriers

SONG Hai-qiong,PANG Guang-chang*,LI Jia-peng   

  1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin
    300134, China
  • Received:2009-04-12 Online:2009-09-01 Published:2014-04-14
  • Contact: PANG Guang-chang E-mail:pgc@tjcu.edu.cn

摘要:

分别以酵母细胞壁微粒、醛基化环糊精微粒(β-CDP)和醛基化淀粉胶材料为载体固定葡萄糖氧化酶(GOD)和β- 半乳糖苷酶(β-GAL)双酶,制作三种乳糖酶膜,并用生物传感分析仪测试它们的性能。实验结果表明,用这三种材料制成的酶膜均表现出优良的性能,在乳糖溶液浓度为0.02%~2% 的范围内具有良好的线性关系,R2 都在0.995 以上,乳糖最低检出浓度可达0.001g/L,具有良好的响应性能,响应时间为20~40s。在这三种酶膜中,酵母壁微粒酶膜的线性范围最宽,达0%~10%,这种传感器可广泛应用于原料乳和乳制品中乳糖的检测;β-CDP酶膜具有较强的吸附酶分子的能力,响应值高达2500;醛基化淀粉胶制成的酶膜材质具有良好的均一性,且制作工艺简单。

关键词: 固定化酶, 酵母细胞壁微粒, 醛基化环糊精微粒, 醛基化淀粉胶, 乳糖酶膜

Abstract:

Glucose oxidase (GOD), together with β-galactosidase (β-GAL), was immobilized onto yeast cell wall particulates, β-cyclodextrin glutaraldehyde particulates (β-CDP) and starch glutaraldehyde adhesive respectively to construct bi-enzyme membranes, whose lactose detection performances were tested using an SBA-40C biosensor. All bi-enzyme membranes performed a good detection capability, with a linear calibration range of 0.02%-2% lactose (R2 above 0.995) and a detection limit of 0.001 g/L and had a fast response, with response time within 20-40s. Among them, bi-enzyme membranes made with yeast cell wall particulates as a carrier had the widest linear calibration range of 0-10% and therefore could be widely used for the detection of lactose in raw milk and dairy products; β-CDP bi-enzyme membranes had a strong adsorption capacity to enzyme molecules and the response values were as high as 2500; bi-enzyme membranes made with starch glutaraldehyde adhesive as a carrier had a good homogeneity and were easy to prepare and could be used to detect lactose in milk.

Key words: immobilization carrier, yeast cell wall particulates, β-cyclodextrin glutaraldehyde particulates (β-CDP), starch glutaraldehyde adhesive, lactose membrane

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